Intracellular condensates are non-membrane bound biochemical subcompartments that form due to phase separation among networks of
weakly interacting molecules. Sabari et al., 2018, proposed that the transcriptional coactivator BRD4 helps form intracellular condensates containing other transcriptional proteins. A prediction of this proposal is that BRD4 should behave as a liquid within the condensate with rapid movement. Which procedure could be used to analyze movement of BRD2 in living cells?
The molecules can move inside and outside of the cell trough the semi permeable membrane. The molecules might require or do not require energy depending upon the concentration gradient.
The FRAP (fluorescence recovery after photobleaching) is the techniques used to identify the diffusion and lateral movement of molecules. This technique uses the fluorescence molecule and then the photobleaching of that particular molecule is directly proportional to the movement of molecule. The bleaching can be observed on the autoradiography.
