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Stolb23 [73]
3 years ago
15

Intracellular condensates are non-membrane bound biochemical subcompartments that form due to phase separation among networks of

weakly interacting molecules. Sabari et al., 2018, proposed that the transcriptional coactivator BRD4 helps form intracellular condensates containing other transcriptional proteins. A prediction of this proposal is that BRD4 should behave as a liquid within the condensate with rapid movement. Which procedure could be used to analyze movement of BRD2 in living cells?
Biology
1 answer:
OLEGan [10]3 years ago
4 0

Answer:

Fluorescence recovery after photobleaching.

Explanation:

The molecules can move inside and outside of the cell trough the semi permeable membrane. The molecules might require or do not require energy depending upon the concentration gradient.

The FRAP (fluorescence recovery after photobleaching) is the techniques used to identify the diffusion and lateral movement of molecules. This technique uses the fluorescence molecule and then the photobleaching of that particular molecule is directly proportional to the movement of molecule. The bleaching can be observed on the autoradiography.

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Two humans mate who are heterozygous for earlobe shape where attached earlobes (A) are dominant to free earlobes (a), and earwax
MariettaO [177]

Answer:

3/16

Explanation:

This question involves two different genes coding for earlobe shape and earwax quality. The alleles for attached earlobes (A) and wet earwax (E) are dominant over the alleles for free earlobes (a) and dry earwax (e).

According to this question, two humans mate who are heterozygous for both genes i.e. AaEe × AeEe. Each parent will produce the following gametes: AE, Ae, aE, ae.

Using these gametes in a punnet square (see attached image), the following proportion of offsprings will be produced:

* Attached earlobe, wet earwax (A_E_) = 9/16

* Attached earlobe, dry earwax (A_ee) = 3/16

* Free earlobe, wet earwax (aaE_) = 3/16

* Free earlobe, dry earwax (aaee) = 1/16

Hence, the probability of these parents having their first child with dry earwax (ee) and attached earlobes (A_) i.e. genotype A_ee is 3/16.

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