Question

What variable did the researchers intentionally vary in the experiment, and what are the units for this variable? what variable did the researchers intentionally vary in the experiment, and what are the units for this variable? inorganic phosphate (inorganic phosphate) concentration, in micromoles per milliliter of sampled buffer glucose 6-phosphate concentration, in micromoles per milliliter of sampled buffer ph, in ph units time of reaction, in minutes?

Answer 1

Answer is: time of reaction, in minutes (data in attachment).
Glucose 6-phosphatase, which is found in mammalian liver cells, is a key enzyme in control of blood glucose levels. The enzyme catalyzes the breakdown of glucose 6-phosphate into glucose and inorganic phosphate. These products are transported out of liver cells into the blood, increasing blood glucose levels. In this exercise, you will graph data from a time-course experiment that measured concentration in the buffer outside isolated liver cells, thus indirectly measuring glucose 6-phosphatase activity inside the cells.