1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
cricket20 [7]
3 years ago
7

The absorbance (????)(A) of a solution is defined as ????=log10(????0????) A=log10⁡(I0I) where ????0I0 is the incident‑light int

ensity and ????I is the transmitted‑light intensity. Absorbance is also defined as ????=????c???? A=ϵcl where ????ϵ is the molar absorption coefficient (extinction coefficient) in units of M−1cm−1,M−1cm−1, cc is the molar concentration, and ????l is path length in centimeters. Daniella prepares a 1 mg/ml1 mg/ml myoglobin solution. The molecular weight of myoglobin is 17.8 kDa.17.8 kDa. Given that the ????ϵ of myoglobin is 15,000 M−1cm−1,15,000 M−1cm−1, calculate the absorbance of the myoglobin solution across a 1 cm1 cm path. Calculate your answer to two decimal places.
Chemistry
1 answer:
bulgar [2K]3 years ago
5 0

Answer:

The absorbance of the myoglobin solution across a 1 cm path is 0.84.

Explanation:

Beer-Lambert's law :

Formula used :

A=\epsilon \times c\times l

A=\log \frac{I_o}{I}

\log \frac{I_o}{I}=\epsilon \times c\times l

where,

A = absorbance of solution

c = concentration of solution

\epsilon = Molar absorption coefficient

l = path length

I_o = incident light

I = transmitted light

Given :

l = 1 cm, c = 1 mg/mL ,\epsilon = 15,000 M^{-1}cm^{-1}

Molar mass of myoglobin = 17.8 kDa = 17.8 kg/mol=17800 g/mol

(1 Da = 1 g/mol)

c = 1 mg /mL = {1mg /mL}{\text{Molar mass of myoglobin}}

c = \frac{1 mg/mL}{ 17800 g/mol} = 5.6179\times 10^{-5} mol/L

1 mg = 0.001 g, 1 mL = 0.001 L

A= 15,000 M^{-1}cm^{-1}\times 5.6179\times 10^{-5} mol/L\times 1 cm

A=0.8426 \approx 0.84

The absorbance of the myoglobin solution across a 1 cm path is 0.84.

You might be interested in
The elements in a row of the the periodic table​
Aleks [24]

Answer:

<em><u>Rows in the periodic table are called periods</u></em>. As one moves from left to right in a given period, the chemical properties of the elements slowly change. <em><u>Columns in the periodic table are called groups</u></em>. Elements in a given group in the periodic table share many similar chemical and physical properties

Explanation:

5 0
2 years ago
If 0.500 mol of each of the following solutes is dissolved in 2.0 L of water, which will cause the greatest increase in the boil
serious [3.7K]

Answer is: D. Na2SO4.

b(solution) = 0.500 mol ÷ 2.0 L.

b(solution) = 0.250 mol/L.

b(solution) = 0.250 m; molality of the solutions.

ΔT = Kf · b(solution) · i.

Kf - the freezing point depression constant.

i - Van 't Hoff factor.

Dissociation of sodium sulfate in water: Na₂SO₄(aq) → 2Na⁺(aq) + SO₄²⁻(aq).

Sodium sulfate dissociates on sodium cations and sulfate anion, sodium sulfate has approximately i = 3.

Sodium chloride (NaCl) and potassium iodide (KI) have Van 't Hoff factor approximately i = 2.

Carbon dioxide (CO₂) has covalent bonds (i = 1, do not dissociate on ions).

Because molality and the freezing point depression constant are constant, greatest freezing point lowering is solution with highest Van 't Hoff factor.

8 0
3 years ago
Read 2 more answers
In the following reaction, which substance is the precipitate? (NH4)2SO4(aq) + Ba(NO3)2(aq) BaSO4(s) + 2NH4NO3(aq
mariarad [96]
Assuming that the reactants are:

(NH4)2SO4 (aq) + Ba(NO3)2 (aq)

and the products are:

BaSO4 (s) + 2NH4NO3 (aq),

then you will have to determine which product is insoluble. You should have access to solubility rules to help you determine this.

According to the solubility rules, the following elements are considered insoluble when paired with SO4:

Sr^2+, Ba^2+, Pb^2+, Ag^2+, and Ca^2+

Therefore, the precipitate will be BaSO4 (s).

5 0
3 years ago
Read 2 more answers
RIGHT ANWSER WILL BE MARKED BRAINLIEST
dybincka [34]

Answer:

11460

Explanation:

4 0
3 years ago
Yo plz help no links like I’m really finna lose it
Rufina [12.5K]

Answer:

a. Solar

Explanation:

Solar energy causes water to evaporate, which is the driving force of the water cycle. Water from the ocean evaporates and comes back down as rain and it gives life to the Earth and so it continues.

7 0
3 years ago
Read 2 more answers
Other questions:
  • Blue vitriol is commonly used in industrial dyeing processes. what is the chemical formula for blue vitriol, whose systematic na
    7·1 answer
  • a sample of cesium metal reacted completely with water, evolving 48.1 ml of dry H2 at 19C and 768 mmHg. what is the equation for
    7·1 answer
  • The positively charged particles found in the nucleus of an atom are called ______.
    6·1 answer
  • PLEASE HELP ME ASAP!!! ILL GIVE BRAINLIEST ITS PLATO!!!
    5·2 answers
  • What three compounds are produced by the combustion of hydrocarbon fuels?
    14·1 answer
  • 4. Explain why 50 g of liquid water at 0°C heats-up more quickly than 50 g of an ice water mixture at 0°C under the same conditi
    13·1 answer
  • What is the bond angle of a methane molecule that contains four hydrogen atoms bonded to one carbon atom?
    9·2 answers
  • Which of the following is NOT an example of potential energy?
    11·1 answer
  • Ions Types of Ions Cation Anion 1. H+ 2.O-2 3.Ba2+ 4.Br- 5.Hg2+ 6.Li+ 7.Ca2+ 8.F- 9.S-2 10.Fe3+
    12·1 answer
  • Iron has a density of 7.86g/cm^3. Could a block of metal with a mass of 18.2g and a volume of 2.56cm^3 be iron? Show work and ex
    7·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!