Answer and Explanation:
The steps of the sliding filament theory are:
Muscle activation: breakdown of energy (ATP) by myosin.
Before contraction begins, myosin is only associated with a molecule of energy (ATP), which myosin breaks down into its component molecules (ADP + P) causing myosin to change shape.
Muscle contraction: cross-bridge formation
The shape change allows myosin to bind an adjacent actin, creating a cross-bridge.
Recharging: power (pulling) stroke
The cross-bridge formation causes myosin to release ADP+P, change shape, and to pull (slide) actin closer to the center of the myosin molecule.
Relaxaction: cross-bridge detachment
The completion of the pulling stroke further changes the shape of myosin. This allows myosin and ATP to bind, which causes myosin to release actin, destroying the cross-bridge. The cycle is now ready to begin again.
The repeated cycling through these steps generates force (i.e., step 2: cross-bridge formation) and changes in muscle length (i.e., step 3: power stroke), which are necessary to muscle contraction.
Answer:
The Lineweaver- Burk plot is representation of Lineweaver-Burk equation of enzyme kinetics in graphical plot that is presented by the Hans Lineweaver and Dean Burk.
In the presence of a non competitive inhibitor shows higher y-intercept than a control in absence of any inhibitor whereas the x intercept is the same as the control that suggests or tells that maximum enzyme activity of the reaction is decreased, Higher Y intercept than controls means that it is same as 1/Vmax. The X intercept indicates that there is no effect on Km of the enzyme reaction.