Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Earth revolves around the sun, keeping doldrums stationary causes the doldrums to be calm winds. Thus, the correct option is C.
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Why are the doldrums so calm?</h3>
The Intertropical Convergence Zone, also known as the doldrums or calms by sailors due to its constant lack of wind, is the area where the northeast and southeast trade winds meet. It encircles the Earth near the thermal equator, however its exact location changes with the seasons.
The sunlight beams down directly on the area around the equator, the Doldrums are caused by solar radiation from the sun. Instead of blowing horizontally, this heating causes the air to warm and climb straight up. As a result, there is little or no wind for weeks at a time.
For more information regarding doldrums, visit:
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It created an opportunity for the mammals to become dominant on earth by wiping out the dinosaurs.
A cell would have 46 chromosomes during metaphase I.
Answer:
To quote another answer I seen from this same question "I would say that the theme which is reinforced in this excerpt is impossibility of certainty - Hamlet is uncertain what he should do next, and he expresses that uncertainty in this soliloquy. "
Explanation:
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