Phytoplankton - zooplankton- small fish -mammal
This is the food chain of the organisms.
From phytoplankton,which are microorganism, they are eaten by zooplankton. Zooplankton are heterotrophic planktons such as jellyfish and forams. Next to this is small fish, they eat those zooplankton. Lastly, the mammals will eat those fish.
Answer:
The nucleus regulates the synthesis of proteins in the cytoplasm through the use of messenger RNA (mRNA). Messenger RNA is a transcribed DNA segment that serves as a template for protein production. It is produced in the nucleus and travels to the cytoplasm through the nuclear pores of the nuclear envelope, which you'll read about below. Once in the cytoplasm, ribosomes and another RNA molecule called transfer RNA work together to translate mRNA in order to produce proteins.
Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
