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Example 1: Two different enzymes are able to catalyze the same reaction, A → B. They both have the same Vmax, but differ their K

luda_lava [24]

Answer: (a) = 0.022mmoles/minute; (b) = 0.00022mmoles/minute; (c) = 0.022mmoles/minute

Explanation:

The Michaelis- Menten equation is used to show the relationship between substrate concentration and reaction rate.

The equation is Vo = Vmax[S]/(Km + [S]

where Vo is the initial velocity, Vmax is the maximum velocity, [S] is the substrate concentration, Km is the Michaelis constant.

(a) For enzyme 1; Vo = 0.002mmoles/minute, Vmax = ?, [S] = 0.1mM, Km =1.0mM; making Vmax subject of the formula;

Vmax = Vo * (Km + [S])/ [S]

Vmax = 0.002*(1.0 + 0.1)/ 0.1

Vmax = 0.022mmoles/minute (Vmax for Enzyme 1&2)

(b) Vo = Vmax[S]/(Km + [S]

Vo = 0.022 * 0.1/(10+0.1) = 0.00022mmoles/minute

(c) Vo = Vmax[S]/(Km + [S]

Vo = 0.022*1000/(1.0+1000) = 0.022mmoles/minute

3 0

3 years ago

How does gel electrophoresis work? Group of answer choices DNA is negatively charged. When an electrical current is passed throu

Anna71 [15]

Answer:

DNA is negatively charged. When an electrical current is passed through a gel, the DNA is repelled by the nearby negative current and pushed across the gel towards the positively charged side.

Explanation:

Electrophoresis has become a standard molecular biology technique. This technique is based on the use of a constant-uniform electric field that enables the separation and identification of DNA fragments with different lengths, which form bands in the electrophoresis gel. Electrophoresis can be used to analyze not only DNA, but also RNA and proteins. During electrophoresis, the fragments of DNA that are negatively charged will migrate towards the positively charged electrode, where the duration of this movement will depend on the size of the DNA fragment, as well as of the pore size in the gel.

7 0

3 years ago

Can a cell replicate without genetic information?<br> true or false?

Helen [10]

Answer:

Without DNA, cells could not reproduce, which would mean the extinction of the species. Normally, the nucleus makes copies of chromosomal DNA, then segments of DNA recombine, and next the chromosomes divide twice, forming four haploid egg or sperm cells.

Explanation:

7 0

2 years ago

Read 2 more answers

Why don’t absorptive surfaces of the small intestine get damaged by the acids from the stomach?

CaHeK987 [17]

The small intestines have a protective lining that is resistant to acids

7 0

3 years ago

What biome does Ohio live in?

Vlada [557]

I am sorry if this below does not help i tryed my best Most states east of the Mississippi River and the states of Missouri, Arkansas and Louisiana are located in the temperate rain forest biome, which is characterized by cold winters and warm, humid summers. Most of the Midwest is within the grasslands, which has a semi-arid climate with warm to hot summers.

Areas in and around the Rocky Mountains are in the alpine biome. Parts of California, Nevada and Arizona are in the desert biome. Oregon, Washington and southern Alaska are within the taiga biome, which features long, severe winters, and short, humid summers. Northern Alaska is a tundra, with long, dark winters that are especially cold. Hawaii is within the tropical rain forest biome, which is warm throughout the year with brief periods of reduced rain.

7 0

3 years ago