A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a stra
ight line with the equation A = 0.0182 x + 0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.234 . A blank solution has an absorbance of 0.055 .
We are given the absorbance from the calibration curve:
A = 0.0182 x + 0.007
A = 0.234 ( It is already corrected, do not need the blank value )
so we can solve for x, the quantity of protein
x = ( 0.234 -0.007 )µg / 0.0182 = 12.5 µg
Note we are given the value for a blank solution but we do not need it for the absorbance of our sample since it is already corrected as stated in the question. If it were not a corrected absorbance ,we will need to correct the absorbance value. The absorbance of the blank is caused by some absorption from the solvent, cuvette, internal reflection, etc.
Thermal energy is defined as the total kinetic energy of all particles in an object. Even though the cup of water has a higher temperature, the bathtub has more thermal energy because it contains much more mass of water compared to the cup.
