Question

A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.0182 x + 0.007 where A is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.234 . A blank solution has an absorbance of 0.055 .

Answer 1

Answer:

12.5 µg

Explanation:

We are given the absorbance from the calibration curve:

A = 0.0182 x + 0.007

A = 0.234  ( It is already corrected, do not need the blank value )

so we can solve for x, the quantity of protein

x =  ( 0.234 -0.007 )µg / 0.0182 = 12.5 µg

Note we are given the value for a blank solution but we do not need it for the absorbance of our sample since it is already corrected as stated in the question. If it were not a corrected absorbance ,we will need to correct the absorbance value. The absorbance of the blank is caused by some absorption from the solvent, cuvette, internal reflection, etc.