Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
False, people with red hair and freckles don’t have any advantages over people who don’t have them
C. Endocrine
Definition, relating to or denoting glands which secrete hormones or other products directly into the blood.
Answer:
Clouds form when the invisible water vapor in the air condenses into visible water droplets or ice crystals. For this to happen, the parcel of air must be saturated, i.e. unable to hold all the water it contains in vapor form, so it starts to condense into a liquid or solid form.
Explanation:
Answer:
see if the strips repel each other when brought close together
Explanation:
Electric fields can exert forces on objects, even if the objects involved are not touching. One way to demonstrate that electric fields exist around charged objects is to observe the effects of their forces at a distance.
Since Ike knows that each strip of tape carries a negative charge, he could see if the strips repel each other when brought close together.
