Answer:
5.43x10⁻⁵ dm³mol⁻¹= Concentration of tyrosine
2.58x10⁻⁵ dm³mol⁻¹= Concentration of tryptophan
Explanation:
Lambert-Beer law is:
A = E×C×l
Where A is measured absorbance, E is absorption coefficient, C is concentration of solution and l is optical path length.
For the result at 240nm, it is possible to write:
0.660 = 2.00x10³ dm³mol⁻¹cm⁻¹× Ctry × 1cm + 1.12x10⁴ dm³mol⁻¹cm⁻¹× Ctyr × 1cm <em>(1)</em>
At 280 nm:
0.221 = 5.40x10³ dm³mol⁻¹cm⁻¹× Ctry × 1cm + 1.50x10³ dm³mol⁻¹cm⁻¹× Ctyr × 1cm <em>(2)</em>
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Thus:
-2.7× (0.660 = 2.00x10³ × Ctry + 1.12x10⁴ × Ctyr) =
-1.782 = -5.40x10³Ctry - 3.024x10⁴Ctyr
0.221 = 5.40x10³ × Ctry + 1.50x10³ × Ctyr
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-1.561 = -28740×Ctyr
<em>5.43x10⁻⁵ dm³mol⁻¹= Ctyr</em>
Replacing this value in (1) or (2) it is possible to find Ctry, that is:
<em>2.58x10⁻⁵ dm³mol⁻¹= Ctry</em>
