What is mass write a five sentence paragraph

which of the following amino acids are used to measure the protein concentration by UV? A. His B. Pro C. Trp D. Phe E. Tyr

Goryan [66]

Answer:

C. Trp D. Phe E. Tyr

Explanation:

The concentration of a protein has a direct relation with absorbance of the protein in a UV spectrophotometer. The formula which relates concentration with absorbance is described as under:

A = ∈ x c x l

where, A = Absorbance

∈ = Molar extinction co-efficient

c = Concentration of absorbing species i.e. protein

l = Path length of light

Tryptophan (Trp), phenylalanine (Phe ) and tyrosine (Tyr) are three aromatic amino acids which are used to measure protein concentration by UV. It is mainly because of tryptophan (Trp), protein absorbs at 280 nm which gives us an idea of protein concentration during UV spectroscopy.

The table depicting the wavelength at which these amino acids absorb and their respective molar extinction coefficient is as under:

Amino acid Wavelength Molar extinction co-efficient (∈)

Tryptophan 282 nm 5690

Tyrosine 274 nm 1280

Phenylalanine 257 nm 570

In view of table above, we can easily see that Molar extinction co-efficient (∈) of Tryptophan is highest amongst all these 3 amino acids that is why it dominates while measuring concentration.

7 0

3 years ago

Round to 4 significant figures. 35.5450

IrinaVladis [17]

Answer:

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Explanation:

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8 0

2 years ago

Read 2 more answers

What are some non examples of biodiversity

zhenek [66]

A monocrop is a non example of biodiversity because it contains only one species, such as all corn, therefore there is very little biodiversity.

8 0

3 years ago

Read 2 more answers

A gas absorbs 10J of heat and is simulataneously comptessed by a constant external pressure of 0.5atm from 4L to2L in volume.Wha

Ne4ueva [31]

Answer:

B. 111 J

Explanation:

The change in internal energy is the sum of the heat absorbed and the work done on the system:

ΔU = Q + W

At constant pressure, work is:

W = P ΔV

Given:

P = 0.5 atm = 50662.5 Pa

ΔV = 4 L − 2L = 2 L = 0.002 m³

Plugging in:

W = (50662.5 Pa) (0.002 m³)

W = 101.325 J

Therefore:

ΔU = 10 J + 101.325 J

ΔU = 111.325 J

Rounded to three significant figures, the change in internal energy is 111 J.

7 0

3 years ago

Why are aldehydes more reactive than ketones?

Simora [160]

Answer:

Aldehydes are typically more reactive than ketones due to the following factors. ... The carbonyl carbon in aldehydes generally has more partial positive charge than in ketones due to the electron-donating nature of alkyl groups. Aldehydes only have one e- donor group while ketones have two.

Explanation:

5 0

3 years ago