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DiKsa [7]
3 years ago
7

What are some drawbacks of electron microscopes? 3. If an object being viewed under the phase-contrast microscope has the same r

efractive index as the background material, how would it appear? +
Physics
1 answer:
gayaneshka [121]3 years ago
7 0

1. <u>Some drawbacks of electron microscopes:</u>

  • Price, size, repair, researcher learning and image artifacts arising from specimen preparation are the major drawbacks.
  • This form of magnification is a massive, burdensome, costly piece of equipment, highly sensitive to vibration and exterior magnetic field.
  • It must be held in an environment that is big enough to contain the microscope, as well as to secure and prevent any unwanted effect on the electrons.
  • Upkeep includes ensuring balanced voltage supplies, electromagnetic coil / lens currents and cool water circulation so that the specimens are not destroyed or damaged by the heat released during the electrons energization process.

2. If an object being viewed under the phase-contrast microscope has the same refractive index as the background material than "it would be difficult to see because the phase contrast microscope amplifies differences in the refractive index".

<u>Explanation:</u>

In order to improve the comparison of transparent and colorless specimens with the light microscopy pictures, thus phase contrast is used. This allows the visualization of cells and cell elements which would be hard to see using a standard light magnification. The phase comparison does not involve the destruction, fixation or staining of cells.

Due to diffraction and scattering phenomena which exist at the edges of these objects, large, extended specimens are also quickly visualized with phase contrast optical. As light transits through one medium to another, the velocity is changed in proportion to the variations in the refractive index between the two media. Therefore, the wave is either increased or decreased in velocity whenever a coherent light wave produced by the oriented microscope filament progresses via a phase specimen with a particular thickness and refractive index.

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5. Emanuel Zacchini, the famous human cannonball of the Ringling Bros. and Barnum and Bailey Circus,
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Explanation:

सिद्ध कीजिए किसी भी बराबर भुजाओं वाले त्रिभुज में उनके सामने के कोण बराबर होते है

3 0
2 years ago
The amount of energy that must be absorbed or lost to raise or lower the temperature of 1 g of liquid water by 1°c _____.
Fiesta28 [93]

Answer:

4.2 J

Explanation:

Specific heat capacity: This is defined as the amount of a heat required to rise a unit mass of a substance through a temperature of 1 K

From specific heat capacity,

Q = cmΔt.............................. Equation 1

Where Q = amount of energy absorbed or lost, c = specific heat capacity of water, m = mass of water, Δt = Temperature rise.

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