Question

Resolution in chromatography is a function of three fundamental parameters. These are

Answer 1

Answer:

Resolution in chromatography is a function of "selectivity, number of plates, and capacity factor".

Explanation:

Resolution is a measure of the separation of 2 peaks of different retention time t in a chromatography divided by the combined widths of the elution peaks.

The resolution is denoted as a numerical value, such as 0.3, 4.0, or 1.0

. Resolution not only  depend on column, but also on the flow-rate and  mobile phase composition, the rate of gradient and the composition and size of  peptides themselves.

$R_{s}=\frac{1}{4} \times \frac{\alpha-1}{\alpha} \times \frac{k}{k+1} \times \sqrt{N_{t h}}$

Where

$R_s$ is the resolution factor.

$\alpha$ is selectivity factor.

K is the retention factor  or the capacity factor.

$N_{th}$  theoretical plate number.