<u>Answer</u>:
Resolution in chromatography is a function of "selectivity, number of plates, and capacity factor".
<u>Explanation</u>:
Resolution is a measure of the separation of 2 peaks of different retention time t in a chromatography divided by the combined widths of the elution peaks.
The resolution is denoted as a numerical value, such as 0.3, 4.0, or 1.0
. Resolution not only depend on column, but also on the flow-rate and mobile phase composition, the rate of gradient and the composition and size of peptides themselves.
Where
is the resolution factor.
is selectivity factor.
K is the retention factor or the capacity factor.
theoretical plate number.