0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
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Explanation:
Molar mass of HBr = 81 g/mol
Molar mass of nitrogen dioxide gas = 46 g/mol
Molar mass of ethane = 30 g/mol
Graham's Law states that the rate of effusion or diffusion of gas is inversely proportional to the square root of the molar mass of the gas. The equation given by this law follows the equation:

So, the gas with least molar mass will effuse out fastest from the container and that is ethane gas.
The formula for average kinetic energy is:

where,
k = Boltzmann’s constant = 
T = temperature = 273.15 K ( at STP)
As we can see from the formula that kinetic energy depends upon only temperature of the gas molecule.
So, from this we can say that all the gas molecules have the same average kinetic energy at this temperature.
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