Answer: Km = 10μM
Explanation: <u>Michaelis-Menten constant</u> (Km) measures the affinity a enzyme has to its substrate, so it can be known how well an enzyme is suited to the substrate being used. To determine Km another value associated to an eznyme is important: <em>Turnover number (Kcat)</em>, which is the number of time an enzyme site converts substrate into product per unit time.
Enzyme veolcity is calculated as:
![V_{0} = \frac{E_{t}.K_{cat}.[substrate]}{K_{m}+[substrate]}](https://tex.z-dn.net/?f=V_%7B0%7D%20%3D%20%5Cfrac%7BE_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D%7D%7BK_%7Bm%7D%2B%5Bsubstrate%5D%7D)
where Et is concentration of enzyme catalitic sites and has to have the same unit as velocity of enzyme, so Et = 20nM = 0.02μM;
To calculate Km:
![V_{0}*K_{m} + V_{0}*[substrate] = E_{t}.K_{cat}.[substrate]](https://tex.z-dn.net/?f=V_%7B0%7D%2AK_%7Bm%7D%20%2B%20V_%7B0%7D%2A%5Bsubstrate%5D%20%3D%20E_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D)
![K_{m} = \frac{E_{t}.K_{cat}.[substrate]-V_{0}*[substrate]}{V_{0}}](https://tex.z-dn.net/?f=K_%7Bm%7D%20%3D%20%5Cfrac%7BE_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D-V_%7B0%7D%2A%5Bsubstrate%5D%7D%7BV_%7B0%7D%7D)

Km = 10μM
<u>The Michaelis-Menten for the substrate SAD is </u><u>10μM</u><u>.</u>
It would be 18 electrons i think
Charges is then there is a negative sign at the right corner of an element it means you have to add, and when it is positive, it means you have to substract
Answer:
A magician I saw once made a painting of a dove come to life. The bird literally flew out of the painting.
That happened 10 years ago and I'm still wondering how that is even possible.
Explanation:
Answer:
5.758 is the density of the metal ingot in grams per cubic centimeter.
Explanation:
1) Mass of pycnometer = M = 27.60 g
Mass of pycnometer with water ,m= 45.65 g
Density of water at 20 °C = d =
1 kg = 1000 g


Mass of water ,m'= m - M = 45.65 g - 27.60 g =18.05 g
Volume of pycnometer = Volume of water present in it = V


2) Mass of metal , water and pycnometer = 56.83 g
Mass of metal,M' = 9.5 g
Mass of water when metal and water are together ,m''= 56.83 g - M'- M
56.83 g - 9.5 g - 27.60 g = 19.7 g
Volume of water when metal and water are together = v

Density of metal = d'
Volume of metal = v' =
Difference in volume will give volume of metal ingot.
v' = v - V


Since volume cannot be in negative .
Density of the metal =d'
=