Answer:
C. Trp D. Phe E. Tyr
Explanation:
The concentration of a protein has a direct relation with absorbance of the protein in a UV spectrophotometer. The formula which relates concentration with absorbance is described as under:
A = ∈ x c x l
where, A = Absorbance
∈ = Molar extinction co-efficient
c = Concentration of absorbing species i.e. protein
l = Path length of light
Tryptophan (Trp), phenylalanine (Phe ) and tyrosine (Tyr) are three aromatic amino acids which are used to measure protein concentration by UV. It is mainly because of tryptophan (Trp), protein absorbs at 280 nm which gives us an idea of protein concentration during UV spectroscopy.
The table depicting the wavelength at which these amino acids absorb and their respective molar extinction coefficient is as under:
Amino acid Wavelength Molar extinction co-efficient (∈)
Tryptophan 282 nm 5690
Tyrosine 274 nm 1280
Phenylalanine 257 nm 570
In view of table above, we can easily see that Molar extinction co-efficient (∈) of Tryptophan is highest amongst all these 3 amino acids that is why it dominates while measuring concentration.
the answer is A......
it is supported by practical evidence and examples. this is the answer because he tried and tested many different ways to see what would happen so he is happy with the conclusion that what he tested is what he gets.
Answer:
the advantage of using Microsoft Excel to create a graph, as opposed to pen and paper is it will help you get the right answer
Explanation:
Answer:
Decomposition reaction
Explanation:
Chemical equation:
2KClO₃ → 2KCl + 3O₂
The given reaction is decomposition reaction.
Decomposition reaction:
It is the reaction in which one reactant is break down into two or more product.
AB → A + B
Synthesis reaction:
It is the reaction in which two or more simple substance react to give one or more complex product.
Single replacement:
It is the reaction in which one elements replace the other element in compound.
AB + C → AC + B
Double replacement:
It is the reaction in which two compound exchange their ions and form new compounds.
AB + CD → AC +BD
