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jeka57 [31]
4 years ago
7

Affinity chromatography is used to purify a protein of interest by capturing it on Select one:

Chemistry
1 answer:
Vladimir [108]4 years ago
3 0

Answer:

c. matrix material with a strongly interacting molecule (very low dissociation constant with target protein) attached.

Explanation:

<em>Affinity chromatography </em>(AC) is based on the immobilization of the protein of interest inside of the column, by means of having a strongly interacting molecule in the matrix.

Strongly interacting means that the complex Protein-Interacting Molecule will not be easily dissociated, this translates into a low dissociation constant.

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What is the density of ammonia (NH3) at 293 K and 0.913 atm?
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Answer:  The density of  Ammonia is 0.648 g/l

Explanation:

Density = Mass/ Volume

Mass of one mole of  Ammonia (NH3) = 17.031g

Volume =?

Using the ideal gas law we can determine the volume.

PV = nRT

P = 0.913 atm, V= ?, n = 1, R = 0.08206 L.atm/K, and T= 293K

Make V the subject of the formular, we then have;

V= nRT/ P = 1 mol x 0.08206 L.atm/ K.mol x 293 / 0.913 atm

               V = 24.04358/ 0.913 = 26.3L

Having gotten the value of Volume in this question, we then go back to solve for density.

Density = Mass/ Volume

                17.031g/ 26.3L = 0.64756 ≈ 0.648 g/l

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