The biuret test is one of the tests for proteins. It can be used to detect peptide linkages. The biuret test is carried out in an alkaline solution. A coordination complex is formed leading to the appearance of a violet color.
Summarily, the biuret method is a colorimetric technique used to test for proteins and peptides. It involves the formation of a purple (violet) complex of Copper salts in alkaline solution.
Hence when the biuret reagent is added to an unknown substance and it turns purple, the unknown substance is a protein
