The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper.
Explanation:
The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. Small Rf values tend to indicate larger, less soluble pigments while the highly soluble pigments have an Rf value near to one.
I think you're talking about histones. Histones are proteins found in eukayotic cell nuclei. Histones are what DNA wrap around so it fits inside the nucleus and helps with the formation of chromosomes.