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Nata [24]
3 years ago
15

While performing the assays for the LDH kinetics experiment, you will pipet 25ul of 250 ug/ml LDH into 975 ul of assay buffer (T

ris, Lactate, and NAD). What is the final concentration of LDH in the assay?
Chemistry
1 answer:
ArbitrLikvidat [17]3 years ago
6 0

Answer:

6.25 μg/mL

Explanation:

When a dilution is made, the mass of the solute is conserved (Lavoiser's law), so the mass pipetted will be the mass in the assay. The mass is the concentration (C) multiplied by the volume (V). If the pipet solution is called 1, and the assay 2:

m1 = m2

C1*V1 = C2*V2

C1 = 250 μg/mL

V1 = 25 μL

V2 = 975 μL + 25 μL = 1000 μL (is the final volume of the assay after the addition of LDH)

250*25 = C2*1000

C2 = 6.25 μg/mL

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Please help me like now please
777dan777 [17]

Answer:

1-1) NaHCO3 + CH3COOH --> NaCH3COO + H2O + CO2

1-2) 0.5 mole of CO2

2-1) 2C4H10 + 13O2 --> 8CO2 + 10H2O

2-2) 4 mol CO2

Explanation:

<u>Question 1</u>

NaHCO3 + CH3COOH --> NaCH3COO + H2O + CO2

<em>To balance the equation, count the number of atoms on both sides of the equation</em>

(1 Na, 1+3+1H, 1+1+1C, 3+2Oxygen) --> (1 Na, 1+1+1C, 3+2H, 2+1+2Oxygen)

<em>Combining the pluses will give you the following</em>

(1 Na, 5H, 3C, 5Oxygen) --> (1 Na, 3C, 5H, 5Oxygen)

<em>Both sides are the same, therefore the chemical equation is balanced (originally). </em>

From the equation, we can see that <u>1 mole of NaHCO3</u> produces <u>1 mole of CO2</u>.

So that means <u>0.5 mole of NaHCO3</u> would produce <u>0.5 mole of CO2</u>.

<u>Question 2</u>

C4H10 + O2 --> CO2 + H2O

<em>Again, count the number of atoms on both sides of the equation</em>

(4C, 10H, 2O) --> (1C, 2H, 3O)     <em>This time left does not equal right side</em>

<em>You now need to find </em><u><em>factors </em></u><em>that can make both sides equal. </em>

C4H10 + O2 --> <u>4</u>CO2 + H2O    <em>Now the C is balanced, let's recount </em>

<em>(4C, 10H, 2Oxygen) --> (4C, 8+1Oxygen, 2H)      H&O is still not balanced</em>

C4H10 + O2 --> 4CO2 + <u>5</u>H2O    <em>Now the H is balanced, let's recount</em>

<em>(4C, 10H, 2Oxygen) --> (4C, 8+5Oxygen, 10H)      O is still not balanced</em>

C4H10 + (<u>13/2</u>)O2 --> 4CO2 + 5H2O    <em>Now the O is balanced</em>

<em>(4C, 10H, 13Oxygen) --> (4C, 13Oxygen, 10H)</em>

<em>But because 13/2 is a fraction, we want to eliminate that by multiplying every reactant and product by 2 (the denominator).</em>

<u>2</u>C4H10 + <u>13</u>O2 --> <u>8</u>CO2 + <u>10</u>H2O    Now it's completely balanced!

<em>(8C, 20H, 28Oxygen) --> (8C, 28Oxygen, 20H)     Yayy! It's balanced.</em>

Now, 2 mol C4H10 produces 8 mol CO2.

So 1 mol C4H10 produces 4 mol CO2.

6 0
2 years ago
Iron (III) oxide reacts with solid carbon in the followed reaction: 2Fe2O3(s) + 3C(s) → 4Fe(s) + 3CO2(g) What mass of Fe2O3 is n
Veseljchak [2.6K]

953.6 g of iron (III) oxide (Fe₂O₃)

Explanation:

We have the following chemical reaction:

2 Fe₂O₃ (s) + 3 C (s) → 4 Fe (s) + 3 CO₂ (g)

We calculate the number of moles of CO₂ by using the following formula:

pressure × volume = number of moles × gas constant × temperature

number of moles = (pressure × volume) / (gas constant × temperature)

number of moles of CO₂ = (2.1 × 100) / (0.082 × 300)

number of moles of CO₂ = 8.54 moles

Taking in account the chemical reaction we devise the following reasoning:

if         2 mole of Fe₂O₃ produces 3 mole of CO₂

then   X moles of Fe₂O₃ produces 8.54 mole of CO₂

X = (2 × 8.54) / 3 = 5.69 moles of Fe₂O₃

number of moles = mass / molar weight

mass =  number of moles × molar weight

mass of Fe₂O₃ = 5.69 × 160 = 953.6 g

Learn more about:

number of moles

brainly.com/question/14111505

#learnwithBrainly

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3 years ago
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Answer:

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Explanation:

Oil or Lipid + Base lead to Glycerol and Soap

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Every morning, Jeremiah uses a blender to make a smoothie for breakfast. Which of the
Studentka2010 [4]

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3 years ago
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Please help, with step by step work
natali 33 [55]

\qquad ☀️\pink{\bf{ {Answer  = \: \:   85.57g }}}

Molar mass of \bf Cu_2O

\qquad \twoheadrightarrow\sf 63.546 \times 2 +16

\qquad \pink{\twoheadrightarrow\bf 143.092 g}

<u>As we know</u>–

1 mol =\bf 6.02×10^{23} formula units

1 mol\bf Cu_2O = 143.092 g = \bf 6.02×10^{23}formula units

Henceforth –

\bf 3.60×10^{23} formula units \bf Cu_2O–

\qquad \sf :\implies \dfrac{143.092 \times3.60×10^{23  }}{6.02×10^{23}}

\qquad \sf :\implies \dfrac{143.092 \times3.60×\cancel{10^{23  }}}{6.02×\cancel{10^{23}}}

\qquad \pink{:\implies\bf 85.57 g}

5 0
2 years ago
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