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3 years ago

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The amount of protein in a sample was measured by the sample's absorbance of light at a given wavelength. Using standards, a bes

t fit line (calibration curve) of absorbance (y-axes) vs. mg protein (x-axes) gave a slope of 0.0163 and an intercept of 0.104.
What is the amount of protein in an unknown sample that has an absorbance of 0.150?

1 answer:

FinnZ [79.3K]3 years ago

5 0

Answer:

Protein Concentration is 2.82mg/L

Explanation:

According to Beer-Lambert's Law, Absorbance is directly proportional to the concentration.

However, the concentration of a solution can be determined from a calibration curve, in which Absorbance is plotted on the y-axis and the Concentration on the x-axis.

Plotting the best line, the equation of line is used

y = mx + c

where y is absorbance = 0.150

m is slope = 0.0163

x is concentration

c is intercept = 0.104

inserting the values from the question

y = mx + c

0.150 = 0.0163x + 0.104

0.0163x = 0.150 - 0.104

0.0163x = 0.046

Divide both sides by 0.0163

0.0163x/0.0163 = 0.046/0.0163

x = 2.82

Concentration of protein = 2.82 mg/L

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g A microwave oven heats by radiating food with microwave radiation, which is absorbed by the food and converted to heat. If the

Sliva [168]

Answer:

The total photons required = 5.19 × 10²⁸ photons

Explanation:

Given that:

the radiation wavelength λ= 12.5 cm = 0.125 m

Volume of the container = 0.250 L = 250 mL

The density of water = 1 g/mL

Density = mass /volume

Mass = Volume × Density

Thus; the mass of the water = 250 mL × 1 g/mL

the mass of the water = 250 g

the specific heat of water s = 4.18 J/g° C

the initial temperature $T_1$ = 20.0° C

the final temperature $T_2$ = 99° C

Change in temperature $\Delta T$ = (99-20)° C = 79 ° C

The heat q absorbed during the process = ms $\Delta T$

The heat q absorbed during the process = 250 g × 4.18 J/g° C × 79° C

The heat q absorbed during the process = 82555 J

The energy of a photon can be represented by the equation :

= hc/λ

where;

h = planck's constant = $6.626 \times 10^{-34} \ J.s$

c = velocity of light = $3.0 \times 10^8 \ m/s$

= $\dfrac{6.626 \times 10^{-34} \times 3.0 \times 10^8}{0.125}$

= $1.59024 \times 10^{-24}$ J

The total photons required = Total heat energy/ Energy of a photon

The total photons required = $\dfrac{82555 J}{1.59024 \times 10^{-24}J}$

The total photons required = 5.19 × 10²⁸ photons

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How many liters of hydrogen are needed to produce 34 grams NH3

polet [3.4K]

You would need 1000 liters

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3 years ago

How many sulfur atoms are there in 3.90 mol of sulfur?

Ilya [14]

<span>In a mole of anything, there are 6.023 x 10^23 units. So, in 3.9 moles of sulfur, there are 3.9 * 6.023 x 10^23 = 23 x 10^23 = 2.3 x 10^24 atoms (keeping only 2 sig figs). Hope I help!!
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3 years ago

100cm³of a solution of potassium hydroxide contains 0.56g of the dissolved solute. What is the molar concentration of this solut

s2008m [1.1K]

Answer:

[KOH] = 0.10M in KOH

Explanation:

Molar Concentration [M] = moles solute/volume solution in liters

moles KOH = 0.56g/56g/mole = 0.01mole

Volume of solution = 100cm³ = 100ml = 0.10 liter

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3 years ago

Omg GUYS I NEED HELPPP

Ilia_Sergeevich [38]

27) Partial pressure of oxygen: 57.8 kPa

29) Final volume: 80 mL

30) Final volume: 8987 L

31) Due to property of water of being polar, ice floats on water

Explanation:

27)

In a mixture of gases, the total pressure of the mixture is the sum of the partial pressures:

$p_T = p_1 + p_2 + ... + p_N$

In this problem, the mixture contains 3 gases (helium, carbon dioxide and oxygen). We know that the total pressure is

$p_T=201.4 kPa$

We also know the partial pressures of helium and carbon dioxide:

$P_{He}=125.4 kPa\\P_{CO_2}=18.2 kPa$

The total pressure can be written as

$p_T=p_{He}+p_{CO_2}+p_{O_2}$

where $p_{O_2}$ is the partial pressure of oxygen. Therefore, we find

$p_{O_2}=p_T-p_{He}-p_{CO_2}=201.4-125.4-18.2=57.8 kPa$

29)

Assuming that the pressure of the gas is constant, we can apply Charle's law, which states that:

"For an ideal gas at constant pressure, the volume of the gas is proportional to its absolute temperature"

Mathematically,

$\frac{V}{T}=const.$

where

V is the volume of the gas

T is the Kelvin temperature

We can re-write it as

$\frac{V_1}{T_1}=\frac{V_2}{T_2}$

Here we have:

$V_1 = 42 mL$ (initial volume)

$T_1=-89^{\circ}C+273=184 K$ is the initial temperature

$T_2=77^{\circ}C+273=350 K$ is the final temperature

Solving for V2, we find the final volume:

$V_2=\frac{V_1 T_2}{T_1}=\frac{(42)(350)}{184}=80 mL$

30)

For this problem, we can use the equation of state for ideal gases, which can be written as

$\frac{p_1 V_1}{T_1}=\frac{p_2 V_2}{T_2}$

where in this problem:

$p_1 = 102.3 kPa$ is the initial pressure

$V_1=1975 L$ is the initial volume

$T_1=25^{\circ}C+273=298 K$ is the initial temperature

$p_2=21.5 kPa$ is the final pressure

$T_2=12^{\circ}C+273=285 K$ is the final temperature

And solving for V2, we find the final volume of the balloon:

$V_2=\frac{p_1 V_1 T_2}{p_2 T_1}=\frac{(102.3)(1975)(285)}{(21.5)(298)}=8987 L$

31)

A molecule of water consists of two atoms hydrogen bond with an atom of oxygen ( $H_2 O$ ) in a covalent bond.

While the molecul of water is overall neutral, due to the higher electronegativity of the oxygen atom, electrons are slightly shifted towards the oxygen atom; as a result, there is a slightly positive charge on the hydrogen side, and a slightly negative charge on the oxygen side (so, the molecules is said to be polar).

As a consequence, molecules of water attract each other, forming the so-called "hydrogen bonds".

One direct consequence of the polarity of water is that ice floats on liquid water.

Normally, for every substance on Earth, the solid state is more dense than the liquid state. However, this is not true for water, because ice is less dense than liquid water.

This is due to the polarity of water. In fact, when the temperature of water is decreased to freezing point and water becomes ice, the hydrogen bondings "force" the molecules to arrange in a lattice structure, so that the molecules become more spaced when they turn into solid state. As a result, ice occupies more volume than water, and therefore it is less dense, being able to float on water.

Learn more about ideal gases:

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#LearnwithBrainly

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3 years ago