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Vinil7 [7]
3 years ago
9

The amount of protein in a sample was measured by the sample's absorbance of light at a given wavelength. Using standards, a bes

t fit line (calibration curve) of absorbance (y-axes) vs. mg protein (x-axes) gave a slope of 0.0163 and an intercept of 0.104.
What is the amount of protein in an unknown sample that has an absorbance of 0.150?
Chemistry
1 answer:
FinnZ [79.3K]3 years ago
5 0

Answer:

Protein Concentration is 2.82mg/L

Explanation:

According to Beer-Lambert's Law, Absorbance is directly proportional to the concentration.

However, the concentration of a solution can be determined from a calibration curve, in which Absorbance is plotted on the y-axis and the Concentration on the x-axis.

Plotting the best line, the equation of line is used

y = mx + c

where y is absorbance = 0.150

           m is slope = 0.0163

            x is concentration

             c is intercept = 0.104

inserting the values from the question

y = mx + c

0.150 = 0.0163x + 0.104

0.0163x = 0.150 - 0.104

0.0163x = 0.046

Divide both sides by 0.0163

0.0163x/0.0163 = 0.046/0.0163

x = 2.82

Concentration of protein = 2.82 mg/L

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Explanation:

A clastic sedimentary rock is a rock that is formed from pre-existing rock materials and minerals. This materials have been transported to their new positions by the agents of denudation.

  1. Clastic sedimentary rocks are made up of rock fragments predominantly.
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Explanation:

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When 20.0 grams of an unknown compound are dissolved in 500. grams of benzene, the freezing point of the resulting solution is 3
Lelu [443]

Answer: 120g/mol

Explanation:

The first step we are to take is to calculate the freezing point depression of the solution.

ΔT(f) = freezing point of pure solvent - freezing point of solution

ΔT(f) = 5.48 - 3.77

ΔT(f) = 1.71°C

Next we are to calculate the molal concentration of the solution using freezing point depression

ΔT(f) = K(f) * m

m = ΔT(f)/K(f)

m = 1.71/5.12

m = 0.333 molal

Now, we calculate the molecular weight of the unknown...

m = 0.333 mol = 0.333 mol X per kg of benzene

moles of X = 0.333 mol of X per kg of benzene * 0.5kg of benzene

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MULTIPLE CHOICE QUESTION
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Answer:

This question will be answered based on general understanding of how a controlled experiment should be:

The answer is: We must make sure we are testing/changing only one variable, and controling others

Explanation:

A controlled experiment is an experiment in which the independent variable is changed for a particular group called the EXPERIMENTAL group and unchanged for another group called the CONTROL group.

In a controlled experiment, only one variable should be tested while the others should be kept constant in order to have an effective result or outcome. This is the case in this question, the type of music or soil should be unchanged for each plant because the experimenter must make sure that he/she is testing/changing only one variable, and controling others.

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