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Vinil7 [7]
3 years ago
9

The amount of protein in a sample was measured by the sample's absorbance of light at a given wavelength. Using standards, a bes

t fit line (calibration curve) of absorbance (y-axes) vs. mg protein (x-axes) gave a slope of 0.0163 and an intercept of 0.104.
What is the amount of protein in an unknown sample that has an absorbance of 0.150?
Chemistry
1 answer:
FinnZ [79.3K]3 years ago
5 0

Answer:

Protein Concentration is 2.82mg/L

Explanation:

According to Beer-Lambert's Law, Absorbance is directly proportional to the concentration.

However, the concentration of a solution can be determined from a calibration curve, in which Absorbance is plotted on the y-axis and the Concentration on the x-axis.

Plotting the best line, the equation of line is used

y = mx + c

where y is absorbance = 0.150

           m is slope = 0.0163

            x is concentration

             c is intercept = 0.104

inserting the values from the question

y = mx + c

0.150 = 0.0163x + 0.104

0.0163x = 0.150 - 0.104

0.0163x = 0.046

Divide both sides by 0.0163

0.0163x/0.0163 = 0.046/0.0163

x = 2.82

Concentration of protein = 2.82 mg/L

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A certain element exists as two different isotopes. 65.0% of its atoms have a mass of 24 amu and 35.0% of its atoms have a mass
Sauron [17]

Answer:

24.7 amu

Explanation:

An isotope is when an element can have different number of neutrons but they have same number of protons.

In order to calculate the average atomic mass with the given information do the following operations:

First change de percentages to fractional numbers, divide by 100.

I like to make a table, to organize all data and I believe is easier to understand.

65/100 = 0.65

35/100 = 0.35

% fraction

65.0 0.65

35.0 0.35

total100.0 1

Now multiply each mass with their corresponding fraction

24 (0.65) = 15.6

26 (0.35) = 9.1

%    fraction uma uma

65.0 0.65 24 15.6

35.0 0.35 26 9.1

total100.0    1          24.7

Finally you add the resulting mass and the units will be in uma.

15.6+9.1 = 24.7

Therefore the average atomic mass of this element will be 24.7 uma.

Check the table in the document attached

Download xlsx
4 0
3 years ago
Which of the following statements is true about the atoms of any element:
aniked [119]
B) They have protons that are identical to the protons of all other elements
3 0
2 years ago
A procedure calls for the bromination of 12 mmol of trans‑cinnamic acid with 12 mmol of Br 2 in glacial acetic acid solvent. Sel
SashulF [63]

Answer: The yield of dibromide product will be approximately one‑half of the expected yield.

Explanation:

6 0
3 years ago
Anyone can help me please
Vlad1618 [11]

x=3

Use the pythagorean theorem:

a^2 + b^2 = c^2

4^2 + x^2 = 5^2

16 + x^2 = 25

x^2 = 25-16

x^2 = 9

√x^2 = √9

x = 3

5 0
3 years ago
How much heat is required to change 25.0 g of water from solid to liquid at 0 oC? Water: ΔHfus = 334 J/g; ΔHvap= 2260J/g
xxMikexx [17]

Answer:

The heat required to change 25.0 g of water from solid ice to liquid water at 0°C  is 8350 J

Explanation:

The parameters given are

The temperature of the solid water = 0°C

The heat of fusion,   = 334 J/g

The heat of vaporization, = 2260 J/g

Mass of the solid water = 25.0 g

We note that the heat required to change a solid to a liquid is the heat of fusion, from which we have the formula for heat fusion is given as follows;

ΔH =  m ×

Therefore, we have;

ΔH =  25 g × 334 J/g = 8350 J

Which gives the heat required to change 25.0 g of water from solid ice to liquid water at 0°C  as 8350 J.

3 0
3 years ago
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