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juin [17]
3 years ago
5

What is the ETD and CID in mass spectrometry? What are the differences between ETD and CID?

Chemistry
1 answer:
insens350 [35]3 years ago
5 0

(ETD) based fragmentation to detect ubiquitination sites in proteins. Our results indicate that ETD can provide alternative fragmentation patterns that allow detection of glY modified lysyl side chains, in particular z+1 fragment ions derived from triply charged precursor ions. We subsequently applied ETD fragmentation-based analysis and detected novel ubiquitination sites on DNA polymerase B1 that were not easily observed using CID. We conclude that ETD can provide significant alternative fragmentation information that complements CID-derived data to improve the coverage when mapping ubiquitination sites in proteins.

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2 years ago
A phosphate buffer solution (25.00 mL sample) used for a growth medium was titrated with 0.1000 M hydrochloric acid. The compone
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Explanation:

sodium monohydrogenphosphate (Na₂HP) and sodium dihydrogenphosphate (NaH₂P) react with HCl thus:

Na₂HP + HCl ⇄ NaH₂P + NaCl <em>(1)</em>

NaH₂P + HCl ⇄ H₃P + NaCl <em>(2)</em>

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0,01862L of HCl×\frac{0,1000mol}{L}= 1,862x10⁻³moles of HCl ≡ moles of phosphate buffer.

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<em></em>

I hope it helps!

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