Answer:
Explanation:
For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.
One way is manager changes itself and the other one is the same thing i think.
Answer:
square cross section. The bar is made of a 7075-T6 aluminum alloy which has a yield strength of 500 MPa, a tensile strength of 575 MPa, and a fracture toughness of 27.5 MPaâm.
Required:
a. What is the nominal maximum tensile stress on the bar?
b. If there were an initial 1.2 mm deep surface crack on the right surface of the bar, what would the critical stress needed to cause instantaneous fast fracture of the bar be?
1.Only suitable for dc
2.more expensive than moving iron type
3. Easily damaged
Answer:
Head loss=0.00366 ft
Explanation:
Given :Water flow rate Q=0.15 
= 6 inch=0.5 ft
=2 inch=0.1667 ft
As we know that Q=AV
So 
=0.687 ft/sec
We know that Head loss due to sudden contraction
If nothing is given then take K=0.5
So head loss
=0.00366 ft
So head loss=0.00366 ft
