Answer:
if you are working with hazardous materials.
Explanation:
A properly operating and correctly used fume hood can reduce or eliminate exposure to volatile liquids, dusts, and mists. It is advisable to use a laboratory hood when working with all hazardous substances.
One of the best buffer choice for pH = 8.0 is Tris with Ka value of 6.3 x 10^-9.
To support this answer, we first calculate for the pKa value as the negative logarithm of the Ka value:
pKa = -log Ka
For Tris, which is an abbreviation for 2-Amino-2-hydroxymethyl-propane-1,3 -diol and has a Ka value of 6.3 x 10^-9, the pKa is
pKa = -log Ka
= -log (6.3x10^-9)
= 8.2
We know that buffers work best when pH is equal to pKa:
pKa = 8.2 = pH
Therefore Tris would be a best buffer at pH = 8.0.
Answer:52M
Explanation:
use S= 1000w/ MV
Here, s= molarity
w= the amount of NaCl
M= the atomic mass of NaCl
V= the volume of solution