A new viral pathogen has emerged, and you have been asked to develop an ELISA-based detection assay to the intact virions. In or
der to ensure that a mutation in a single surface protein does not render your assay obsolete, which of the following should you use? a. Fluorescence in situ hybridization (FISH)
b. Molecular beacons
c. Monoclonal antibodies
d. Polyclonal antibodies
e. Subunit vaccine
The correct answer is option d. "Polyclonal antibodies".
Explanation:
The enzyme-linked immunosorbent assay (ELISA) is very useful in comparison to other immunoassay methods for being highly accurate, sensitive and specific. Polyclonal antibodies for the detection step could be used on order to ensure that a mutation in a single surface protein would not make obsolete an ELISA used to detect a new viral pathogen. Unlike monoclonal antibodies, polyclonal antibodies are a collection of antibodies that recognize multiple epitopes of the same antigen. Therefore, a mutation in one epitope that affects the binding of one antibody would not affect the bind of the others and the ELISA will be able to recognize the pathogen.
Euglena are single cell flagellates....they have chloroplasts so are capable of photosynthesis- like a plant...but can also “eat” other carbon sources. They belong to the Kingdom Protista.
