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Bumek [7]
3 years ago
8

You have recovered two LacZ-minus AmpR colonies from your transformation in Lab 7 and they appear as white colonies on a LBAX pl

ate. You test each colony on a LBT plate. Only one of these two colonies grew on the LBT plate (Colony 1) while the other colony did not grow on the LBT plate (Colony 2). What is most likely the plasmid contained in Colony 2?
A: pUC119
B: pACYC184
C: pUC-tet(resistant)
D: pUC-chloramphenicol(minus)
Chemistry
1 answer:
svlad2 [7]3 years ago
7 0

Answer:

D. pUC-chloramphenicol(minus)

Explanation:

It contains chloramphenicol resistance gen, the PMB1 posses origin of replication (ori), beta-galactosidase coding gen Laz. It also has pUC18 with many cloning site in the Lac Z gene which makes the recombinant clones to be verified via culture plates which is made up of IPTG and X- Gal.

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This is the question pls help me
Kryger [21]

Answer:  B

Explanation:  Graph B compares the two temperatures on separate lines so that we can see the comoparison directly, as a function of time.  Not only does the graph quickly answer which condition id most favorable to colony growth, but it also hints at some behaviors that may accelerate growth as time goes on.  Graph C is a possible answer, if the <u>only</u> question is which promotes growth the fastest.   But the questions asks "compare," which Graph B does not allow as well as Graph C.

4 0
3 years ago
A scientist wants to perform a controlled experiment to test the effect of rust on chemically treated iron. which action would b
olga2289 [7]
The answer is to test a piece of untreated and treated under same conditions!
9 0
3 years ago
Read 2 more answers
A temperature of 37C is equivalent to a temperature of
ss7ja [257]
Answer 98.6 Fahrenheit
8 0
3 years ago
Read 2 more answers
Match each statement with a layer of the atmosphere. There will be two statements for each layer.
LuckyWell [14K]

<u>The troposphere: </u>

H. This layer can have thunderstorms or clear, sunny skies.

A. The biosphere interacts most with this layer. 

<u>The stratosphere:</u>

B. It is the second layer from Earth's surface. 

G. Winds are strong and steady in this layer. 

<u>The mesosphere:</u>

E. It is heated by the ozone layer beneath it. 

D. This layer is where most meteor showers occur. 

<u>The thermosphere :</u>

F. It contains the ionosphere and exosphere. 

C. It contains layers of single, unmixed gas. 

<u>Explanation:</u>

Depending on the Earth's  temperature the atmosphere can be separated into layers. The troposphere, the stratosphere, the mesosphere and the thermosphere are those layers. The lowest layer is named as Troposphere (0-10 km from the Earth outer surface), it comprises about 75% of the atmosphere's total air and nearly most the water vapor.

Stratosphere (10-30) includes much of the surface ozone. The change in height temperature arises as this ozone absorbs ultraviolet (UV) radiation from the sun. The temperature in Mesosphere (30-50 Km) declines again with height, hitting a minimum of about -90 ° C at the "mesopause." Above this thermosphere (50-400 Km) is settled which is a area where temperatures rise with height once again. The penetration of intense  UV and X-ray radiation from the sun induces this temperature rise.

6 0
3 years ago
For a tablet containing 500. mg of vitamin C, calculate how many ml of 0.095 M NaOH is required to reach the equivalence point.
Gala2k [10]

Answer:

mL of NaOH required =29.9mL

Explanation:

Let us calculate the moles of vitamin C in the tablet:

The molar mass of Vitamin C is 176.14 g/mole

moles=\frac{mass}{molarmass}=\frac{500mg}{176.14}=\frac{0.5}{176.14}=0.0028

Thus we need same number of moles of NaOH to reach the equivalence point.

For NaOH solution:

moles=MolarityXvolume=0.095Xvolume

0.00283=0.095Xvolume

volume=0.0299L=29.9mL

7 0
3 years ago
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