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Bumek [7]
3 years ago
8

You have recovered two LacZ-minus AmpR colonies from your transformation in Lab 7 and they appear as white colonies on a LBAX pl

ate. You test each colony on a LBT plate. Only one of these two colonies grew on the LBT plate (Colony 1) while the other colony did not grow on the LBT plate (Colony 2). What is most likely the plasmid contained in Colony 2?
A: pUC119
B: pACYC184
C: pUC-tet(resistant)
D: pUC-chloramphenicol(minus)
Chemistry
1 answer:
svlad2 [7]3 years ago
7 0

Answer:

D. pUC-chloramphenicol(minus)

Explanation:

It contains chloramphenicol resistance gen, the PMB1 posses origin of replication (ori), beta-galactosidase coding gen Laz. It also has pUC18 with many cloning site in the Lac Z gene which makes the recombinant clones to be verified via culture plates which is made up of IPTG and X- Gal.

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Molar mass is the ratio of the mass to that amount of the substance. The mass of the barium nitrate in the formula unit is 23.0 grams.

<h3>What is mass?</h3>

The mass of a substance is the product of the molar mass of the compound and the number of moles of the compound.

Given,

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If, 6.022 \times 10^{23} have a mass of 261.35 g/mol then, 5.30 \times 10^{22} formula units will have a mass of,

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