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Bumek [7]
3 years ago
8

You have recovered two LacZ-minus AmpR colonies from your transformation in Lab 7 and they appear as white colonies on a LBAX pl

ate. You test each colony on a LBT plate. Only one of these two colonies grew on the LBT plate (Colony 1) while the other colony did not grow on the LBT plate (Colony 2). What is most likely the plasmid contained in Colony 2?
A: pUC119
B: pACYC184
C: pUC-tet(resistant)
D: pUC-chloramphenicol(minus)
Chemistry
1 answer:
svlad2 [7]3 years ago
7 0

Answer:

D. pUC-chloramphenicol(minus)

Explanation:

It contains chloramphenicol resistance gen, the PMB1 posses origin of replication (ori), beta-galactosidase coding gen Laz. It also has pUC18 with many cloning site in the Lac Z gene which makes the recombinant clones to be verified via culture plates which is made up of IPTG and X- Gal.

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Compute the freezing point of this solution:
likoan [24]

Answer:

Freezing point = 1.25

Explanation:

If  we increase the concentration of the solution, the concentration of H+ does not change.

Convert 2.5% in to decimal

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8 0
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Describe each highlighted bond in terms of the overlap of atomic orbitals. (If the highlighted bond is not a pi bond, select the
Anestetic [448]

The image of the bonds are missing, so i have attached it.

Answer:

A) - Sigma bond

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- None

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Explanation:

A) For compound 1;

- the molecular orbital type is sigma bond due to the end-to-end overlapping.

- Atomic orbitals in the sigma bond will be Sp³ and Sp³

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B) For compound 2;

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6 0
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What is generally the best approach when liquid from a large reagent bottle is needed in an experiment
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Answer:

See the answer below

Explanation:

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6 0
4 years ago
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