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Bumek [7]
3 years ago
8

You have recovered two LacZ-minus AmpR colonies from your transformation in Lab 7 and they appear as white colonies on a LBAX pl

ate. You test each colony on a LBT plate. Only one of these two colonies grew on the LBT plate (Colony 1) while the other colony did not grow on the LBT plate (Colony 2). What is most likely the plasmid contained in Colony 2?
A: pUC119
B: pACYC184
C: pUC-tet(resistant)
D: pUC-chloramphenicol(minus)
Chemistry
1 answer:
svlad2 [7]3 years ago
7 0

Answer:

D. pUC-chloramphenicol(minus)

Explanation:

It contains chloramphenicol resistance gen, the PMB1 posses origin of replication (ori), beta-galactosidase coding gen Laz. It also has pUC18 with many cloning site in the Lac Z gene which makes the recombinant clones to be verified via culture plates which is made up of IPTG and X- Gal.

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Which macromolecules are polymers made of nucleotides
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3 years ago
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A pan containing 40 grams of water was allowed to cool from a temperature of 91.0C. If the amount of heat repressed is 1,300 jou
Vinvika [58]

Answer:

83°C

Explanation:

The following were obtained from the question:

M = 40g

C = 4.2J/g°C

T1 = 91°C

T2 =?

Q = 1300J

Q = MCΔT

ΔT = Q/CM

ΔT = 1300/(4.2x40)

ΔT = 8°C

But ΔT = T1 — T2 (since the reaction involves cooling)

ΔT = T1 — T2

8 = 91 — T2

Collect like terms

8 — 91 = —T2

— 83 = —T2

Multiply through by —1

T2 = 83°C

The final temperature is 83°C

3 0
3 years ago
If 3.00 mL of 0.0250 M CuSO4 is diluted to 25.0 mL with pure water, what is the molarity of copper(II) sulfate in the diluted so
g100num [7]

Answer:

0.00268 M

Explanation:

To find the new molarity, you need to (1) find the moles of CuSO₄ (via the molarity equation using the beginning molarity and volume) and then (2) find the new molarity (using the moles and combined volume). Your final answer should have 3 sig figs to match the given values.

<u>Step 1:</u>

3.00 mL / 1,000 = 0.00300 L

Molarity = moles / volume (L)

0.0250 M = moles / 0.00300 L

(0.0250 M) x (0.00300 L) = moles

7.50 x 10⁻⁵ = moles

<u>Step 2:</u>

25.0 mL / 1,000 = 0.0250 L

0.0250 L + 0.00300 L = 0.0280 L

Molarity = moles / volume (L)

Molarity = (7.50 x 10⁻⁵ moles) / (0.0280 L)

Molarity = 0.00268 M

8 0
1 year ago
Select the options that are properties of electromagnetic waves:
kaheart [24]

Answer:

They propagate in materialistic media and non-materialistic media ( space ) .

They propagate in the space at constant velocity , which is 3 × 108 m/s .

They consist of vibrating electric and magnetic fields at certain frequency in phase with each other , perpendicular to each other and perpendicular to the direction of the wave propagation ...

Explanation:

welcome!

4 0
3 years ago
Cual es la relación de la temperatura y la solubilidad?
DanielleElmas [232]

Answer:

A medida que aumenta la temperatura de un líquido, la solubilidad de los gases en ese líquido disminuye. Podemos usar la Segunda Ley de la Termodinámica para explicar por qué. Calentar una solución de un gas permite que las partículas de gas se muevan más libremente entre la solución y la fase gaseosa. La Segunda Ley predice que cambiarán al estado más desordenado, más altamente disperso y, por lo tanto, más probablemente gaseoso.

Explanation:

8 0
3 years ago
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