Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Resident microflora b. may be responsible for opportunistic infections in immunocompromised
Explanation:
It is almost impossible to found these microorganisms in the stomach because of the acidic conditions, most of them are not adapted to this type of environment. Resident microflora is not transient, is actually permanent. However, resident microflora may be responsible for ooportunistic infections in immunocompromised and they can also increase the intensity of the disease produced by that pathogen.
Answer: The thoracic cavity contains the heart, lungs and many of the main vessels of the circulatory system. It is found anterior to the vertebral cavity.
Explanation:
The thoracic cavity is the second largest hollow space of the body and it is the space limited by the ribs, the vertebral column, and the sternum. It is the upper region of the body starting from the neck and ending where the diaphragm ends. So, it is separated from the abdominal cavity by the diaphragm. It contains 12 ribs, and 7 of them attach directly to the sternum, 3 attach to the sternum via cartilages and 2 do not attach to the sternum at all and are called floating ribs. The cavity contains several organs, such as the lungs, heart, great vessels and esophagus.
The abdominal cavity spaces around the organs are filled with the core muscles that attach to the posterior spine. Besides. the ribs vertically, horizontally and across the abdominal region.
The vertebral cavity is the posterior portion of the dorsal cavity, it contains the spinal cord within the vertebral column, the meninges and the fluid-filled spaces between them. It is formed by the vertebrae through which the spinal cord passes.
<u>The thoracic cavity is anterior to the vertebral cavity, because anterior describes the front or direction toward the front of the body</u>.
Answer:
22 pairs of autosomes, 1 pair of sex chromosomes, I notice that the X and Y chromosomes are sex chromosomes and that they are different in length and size., The sex of this organism is male, as the genotype XY can only mean a male gender; female gender is denoted by the genotype XX., You didn't put in Karyotype A., The special circumstance is with group 21 of chromosomes. There are 3 instead of 2 in that group., This circumstance results in a condition known as trisomy 21, or Down Syndrome. Down syndrome causes a distinct facial appearance, intellectual disability, developmental delays, and may be associated with thyroid or heart disease.
, Name the karyotype "Down Syndrome Karyotype?" (I'm not sure on the last one)
Answer:
to be thorough and pay attention to detail.
the ability to use your initiative.
to be flexible and open to change.
patience and the ability to remain calm in stressful situations.
the ability to work well with others.
the ability to accept criticism and work well under pressure.
To be successful as an animal breeder, you should have knowledge of the animal breeding process
a passion for animal breeding,
and knowledge of the potential risks of animal breeding.
Ultimately, a top-notch animal breeder should be an effective communicator, have good organizational skills, and be physically fit.
