Question

g In animal tissues the rate of conversion of pyruvate to acetyl-CoA is regulated by the ratio of phosphorylated and dephosphorylated PDH complex. Describe what happens to this reaction when a preparation of rabbit muscle mitochondria containing PDH complex is treated with:

Answer 1

Answer: seen below.

Explanation: since the different means use in treating rabbit muscle mitochondria containing PDH complex. I will give different reactions that occurs.

The mitochondria preparation responds as follow;

Active pyruvate dehydrogenase (dephosphorylated) is converted to inactive pyruvate dehydrogenase (phosphorylated) and the rate of conversion of pyruvate to acetyl-CoA decreases.

The phosphoryl group on pyruvate dehydrogenase (dephosphorylated) phosphate is removed enzymatically to give active pyruvate dephosphorylated which increases the rate of conversion of pyruvate to acetyl-CoA.

Malonate inhibit succinate dehydrogenase, and citrate accumulates. Accumulation of this citrate inhibits citrate synthase, and acetyl-CoA accumulates. Increased level of this acetyl-CoA inhibits pyruvate dephosphorylated and the rate of conversion of pyruvate to acetyl-CoA decreases.