Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
Answer:
the oxidation state od boron in sodium boron hydride is (+3).
A bimolecular reaction is always a second-order reaction, but a second-order reaction is not always a bimolecular reaction.
The most important thing to take note of is that molecularity of a reaction is a concept applicable to only elementary reactions, meaning non-complex. In a way, elementary reactions are basic and achieved in one step. Complex reactions involve intermediate steps before achieving the desired reaction.
Molecularity is equal to the sum of the coefficients of the reactants, so two reactants give a second-order bimolecular reaction. However, second-order reactions can involve more than two reactants especially in complex reactions.
Answer:
The pH of a solution with the [H + ] = 1 × 10 -2 M is 2 and the pH of a solution with the [H + ] = 1 × 10 -10 M is 10. ( I think this is correct ).
Explanation:
