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MatroZZZ [7]
3 years ago
12

Ampicillin is a bacteriostatic antibiotic. (Instead of killing bacteria, it inhibits their growth.) Ramon plated bacteria transf

ormed with a plasmid that confers ampicillin resistance. He plated the bacteria on Thursday and left them in a 37 oC incubator overnight. On Friday, he observed moderately sized bacterial colonies. He decide to leave them in the incubator a little longer before picking the colonies that he wanted to work with further. Unfortunately, he forgot about the plate and left it in the incubator over the weekend. On Monday, his plate had large colonies that were each surrounded by very small colonies
A) Interpret and explain Ramon’s observations.

B) Predict what he will find when he picks some large and some small colonies and follows the plasmid isolation protocol for each of the colonies.
Chemistry
1 answer:
love history [14]3 years ago
3 0

Answer:

A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.

B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.

On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.

Explanation:

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4 years ago
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3 years ago
Dr. Patel and her team have been using GPS to track two plates that are moving toward each
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C: One plate is going underneath the other plate and sinking into the soft rock below.

Explanation:

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7 0
3 years ago
How many grams of oxygen can be obtained from 250. grams of KCIO3?
guapka [62]

Answer:

Keep it simple. If all the oxygen contained in the 200 grams of potassium chlorate is produced in the decomposition, then all we have to do is find out how many grams of oxygen are there in the 200 grams. This we can do by calculating the ratio of oxygen mass to the whole. Using 39.1 for potassium, 35.45 for chlorine and 3 times 16, or 48 for the oxygen, we get a total of 122.55 grams per mole for potassium chlorate, of which 48 grams are oxygen. This ratio is 48/122.55. This ratio times the original 200 grams of the compound, gives us 78.34 grams of oxygen produced.

Explanation:

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2 years ago
How did the surface area of the metal strip expose to the solution affect the
frez [133]

Answer:

See explanation below

Explanation:

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3 years ago
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