1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
MatroZZZ [7]
3 years ago
12

Ampicillin is a bacteriostatic antibiotic. (Instead of killing bacteria, it inhibits their growth.) Ramon plated bacteria transf

ormed with a plasmid that confers ampicillin resistance. He plated the bacteria on Thursday and left them in a 37 oC incubator overnight. On Friday, he observed moderately sized bacterial colonies. He decide to leave them in the incubator a little longer before picking the colonies that he wanted to work with further. Unfortunately, he forgot about the plate and left it in the incubator over the weekend. On Monday, his plate had large colonies that were each surrounded by very small colonies
A) Interpret and explain Ramon’s observations.

B) Predict what he will find when he picks some large and some small colonies and follows the plasmid isolation protocol for each of the colonies.
Chemistry
1 answer:
love history [14]3 years ago
3 0

Answer:

A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.

B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.

On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.

Explanation:

You might be interested in
The following data is given to find the formula of a Hydrate:
umka21 [38]

The masses can be found by substractions:

  • Mass of CaSO₄.H2O (hydrate):

16.05 g - 13.56 g = 2.49 g

  • Mass of CaSO₄ anhydrate:

15.07 g - 13.56 g = 1.51 g

  • The mass of water is equal to the difference between the mass of the hydrate and the mass of the anhydrate:

2.49 g - 1.51 g = 0.98 g

  • The percent of water is found by the formula:

massWater ÷ massHydrate * 100%

0.98 g ÷ 2.49 g * 100% = 39.36%

  • The mole of water is calculated using water's molecular weight (18g/mol):

0.98 g ÷ 18 g/mol = 0.054 mol water

  • A similar procedure is made for the mole of salt (CaSO₄ = 136.14 g/mol)

1.51 g ÷ 136.14 g/mol = 0.011 mol CaSO₄

  • The ratio of mole of water to mole of anhydrate is:

0.054 mol water / 0.011 mol CaSO₄ = 0.49

In other words the molecular formula for the hydrate salt is CaSO₄·0.5H₂O

3 0
3 years ago
A student placed equal volumes of honey and of water in two, identical, open dishes, and left them at room temperature for 8 hou
Ahat [919]

Answer: Honey has a much lower vapor pressure than pure water has. So, pure water evaporates at a much higher rate.

Explanation:

8 0
3 years ago
Read 2 more answers
How do you prepare 1 liter of a 0.5 molar glucose solution?
riadik2000 [5.3K]
Answer: 90 grams

0.5 molar of glucose means 0.5mole glucose for 1 liter of water. Since we want to made 1L of solution, then the amount of glucose needed is: 0.5mole/l x 1l= = 0.5mole

Glucose molecular weight is 180, then 0,5 mol of glucose is= 0.5mole x 180 grams/mole= 90 grams.
6 0
3 years ago
How many liters of P, are in 3.5x1022 molecules at STP?
suter [353]

Answer: they giving you some hard question

Explanation:

i dont know  know what the answer is do you have any answer options

4 0
3 years ago
Method that used to separate kerosine and water?
nata0808 [166]
You can use a separating funnel. The person will go the top and the water to the bottom.
7 0
3 years ago
Read 2 more answers
Other questions:
  • If 8cm^3 of H2 reacts with an excess of Cl2, calculate how much of the HCL(g) is produced.
    10·1 answer
  • Which of the following statements is true?
    6·1 answer
  • In a solution of salt and water blank is the solvent
    8·1 answer
  • Find the number of formula units in 30 moles of h3po4
    11·1 answer
  • State the Periodic Law. Why is it so important in the study of chemistry?
    10·1 answer
  • 3. What is the substance ozone?
    5·1 answer
  • A woman is found dead on a boat floating in the middle of a lake at 4:30am. There is blunt force trauma to her head but no other
    5·1 answer
  • A chemist adds of a magnesium fluoride solution to a reaction flask. Calculate the mass in micrograms of magnesium fluoride the
    11·1 answer
  • Click on the event or events that led to the formulation of the cell theory
    15·1 answer
  • A sample of an unknown gas occupies 5.51 L at 1.31 atm. What pressure would thisgas exert in a 0.520 L container if the temperat
    7·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!