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MatroZZZ [7]
3 years ago
12

Ampicillin is a bacteriostatic antibiotic. (Instead of killing bacteria, it inhibits their growth.) Ramon plated bacteria transf

ormed with a plasmid that confers ampicillin resistance. He plated the bacteria on Thursday and left them in a 37 oC incubator overnight. On Friday, he observed moderately sized bacterial colonies. He decide to leave them in the incubator a little longer before picking the colonies that he wanted to work with further. Unfortunately, he forgot about the plate and left it in the incubator over the weekend. On Monday, his plate had large colonies that were each surrounded by very small colonies
A) Interpret and explain Ramon’s observations.

B) Predict what he will find when he picks some large and some small colonies and follows the plasmid isolation protocol for each of the colonies.
Chemistry
1 answer:
love history [14]3 years ago
3 0

Answer:

A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.

B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.

On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.

Explanation:

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I need help with this question
Maru [420]

Aluminum sulfide : Al₂S₃

ratio cation : anion = 2 : 3

<h3>Further explanation</h3>

Given

Compound of Aluminium

Required

cations anions ratio

Solution

Salt can be formed from cations and anions which have their respective charges.

In the chemical compound formula these charges are crossed with each other

For aluminum it has a +3 charge

1. Aluminum carbide : Al₄C₃

ratio cation : anion = 4 : 3

2. Aluminum chloride : AlCl₃

ratio cation : anion = 1 : 3

3. Aluminum sulfide : Al₂S₃

ratio cation : anion = 2 : 3

4. Aluminum nitride : AlN

ratio cation : anion = 1 : 1

3 0
2 years ago
Suppose that 100 grams of water at 50.0°C is placed in contact with 200 grams of iron at 30.0°C. The final
wel

Answer:

The answer would be 1.5 kJ.

Explanation:

When you use the equation q = m x c x ∆T you will be able to find the energy gained or lost. The data for the water in this case is just there to distract you so ignore it. :D

4 0
2 years ago
The equilibrium constant for the reaction AgBr(s) Picture Ag+(aq) + Br− (aq) is the solubility product constant, Ksp = 7.7 × 10−
barxatty [35]

Answer:

The reaction will be  non spontaneous at these concentrations.

Explanation:

AgBr(s)\rightarrow Ag^+(aq) + Br^- (aq)

Expression for an equilibrium constant K_c:

K_c=\frac{[Ag^+][Br^-]}{[AgCl]}=\frac{[Ag^+][Br^-]}{1}=[Ag^+][Br^-]

Solubility product of the reaction:

K_{sp}=[Ag^+][Br^-]=K_c=7.7\times 10^{-13}

Reaction between Gibb's free energy and equilibrium constant if given as:

\Delta G^o=-2.303\times R\times T\times \log K_c

\Delta G^o=-2.303\times R\times T\times \log K_{sp}

\Delta G^o=-2.303\times 8.314 J/K mol\times 298 K\times \log[7.7\times 10^{-13}]

\Delta G^o=69,117.84 J/mol=69.117 kJ/mol

Gibb's free energy when concentration [Ag^+] = 1.0\times 10^{-2} M and [Br^-] = 1.0\times 10^{-3} M

Reaction quotient of an equilibrium = Q

Q=[Ag^+][Br^-]=1.0\times 10^{-2} M\times 1.0\times 10^{-3} M=1.0\times 10^{-5}

\Delta G=\Delta G^o+(2.303\times R\times T\times \log Q)

\Delta G=69.117 kJ/mol+(2.303\times 8.314 Joule/mol K\times 298 K\times \log[1.0\times 10^{-5}])

\Delta G=40.588 kJ/mol

  • For reaction to spontaneous reaction:  \Delta G.
  • For reaction to non spontaneous reaction:  \Delta G>0.

Since ,the value of Gibbs free energy is greater than zero which means reaction will be non spontaneous at these concentrations

5 0
3 years ago
Why don't solids change shape?
borishaifa [10]

Answer: B) the forces in the solid hold the atoms tightly in place

Explanation:This is because there is no room for the atoms to move around much and because they are very close together which makes the phase a solid.

6 0
3 years ago
Write the formula and determine the percent by mass of the salt in barium hydroxide octahydrate *
user100 [1]

Answer:

315.51g/mol

Explanation:

137(33 + (16.00 + 1.01) 2 + 8 [1.01 (2) + 16.00] = 315.51g/mol

7 0
2 years ago
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