Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
Answer:
B. Mitochondrion
Explanation:
The Krebs Cycle occurs in the mitochondria during the processes of cellular respiration.
Specifically, it occurs in the matrix of the mitochondria.
The answer is; A
When an impulse from the motor neuron reached the neuromuscular junction, the voltage-dependent calcium channels are activated and the neurotransmitter released from the presynaptic cleft. When the neurotransmitter binds to their receptors on the sarcolemma, the muscle fibers become depolarised; the calcium is released from their vesicles. The Ca2+ ions are important in the power stroke because they bind to troponin. Upon binding calcium, troponin moves tropomyosin away from the myosin-binding sites on actin. Powered by the energy from ATP molecule, the myosin is able to bind on the actin and slides over the actin filament.
