I think the awnser to your question is C
        
             
        
        
        
Answer: Contamination
Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.
 
        
             
        
        
        
The balanced reaction is:<span>
Mg (s) + 2HCl (aq)
--> MgCl 2 (aq) + H 2 (g) 
We
are given the amount hydrochloric acid to be used for the reaction. This will
be the starting point of the calculation.
40.0 g
HCl ( 1 mol HCl / 36.46 g HCl)
(1 mol H2 / 2 mol HCl) (2.02 g H2 / 1 mol H2) = 1.11 g H2</span>
 
        
             
        
        
        
The final volume of the diluted acid that was initially in a 55 mL bottle of 12 M HCl and diluted with water to a 2.5 M HCl solution is 264mL. 
<h3>How to calculate volume?</h3>
The volume of a solution can be calculated using the following formula:
C1V1 = C2V2
Where;
- C1 = initial concentration
- C2 = final concentration
- V1 = initial volume
- V2 = final volume
12 × 55 = 2.5 × V2
660 = 2.5V2
V2 = 264mL
Therefore, the final volume of the diluted acid that was initially in a 55 mL bottle of 12 M HCl and diluted with water to a 2.5 M HCl solution is 264mL. 
Learn more about volume at: brainly.com/question/1578538
#SPJ1
 
        
             
        
        
        
There are 6 electrons in the atom of a carbon.
The first atomic level will be occupied by 2 electrons while the second will be occupied by 4 electrons.
Based on this, the outer valence shell of carbon (which is L shell) will be occupied by 4 electrons.