Question

If you want to use a serial dilution to make a 1/50 dilution. The first dilution you make is a 1/5 dilution with a total volume of 1 ml. To make that dilution you would add ____of your stock solution and____ of your solvent. Then you make a 1/10 dilution and add ____ of your first dilution and ____ of your solvent.

Answer 1

Answer:

0.2 mL stock solution, 0.8 solvent, 0.1 mL first solution and 0.9 solvent

Explanation:

The final volume for fist solution is 1 mL and concentration must will be 1/5, then 1 mL/5=0.2 mL. For complete the 1 mL add the missing solvent volume 1 mL-0.2 mL=0.8 mL. For second solution, assuming final volume is 1 mL, and concentration 1/10, then we have 1 mL /10=0.1 mL solution 1/5. Completing volume, 1 mL-0.1 mL= 0.9 mL solvent.