What assumptions do we make in order to use the Henderson-Hasselbalch equation? a. Both the weak acid and its conjugate base are
present initially. b. x (or the change in the reaction) is approximately zero, that is we use the weak acid approximation. c. The final and initial concentration of the acid and it's conjugate base are approximately equal, that is we use the weak acid approximation. d. We only have the weak acid initially.
Volume of Hydrogen V1 = 351mL Temperature T1 = 20 = 20 + 273 = 293 K Temperature T2 = 38 = 38 + 273 = 311 K We have V1 x T2 = V2 x T1 So V2 = (V1 x T2) / T1 = (351 x 311) / 293 = 372.56 Volume at 38 C = 373 ml