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LenaWriter [7]
3 years ago
11

Indicate which of the following criteria are important for the selection of a buffer to use in an in vitro biochemical reaction.

(Select all that apply.)
a. The molecular weight of the chosen buffer should be in the range of 150 g/mol to 650 g/mol.
b. The chosen buffer should not interfere with the biochemical reaction.
c. The makeup of the chosen buffer should include only carbon, hydrogen, nitrogen, oxygen, and phosphorus.
d. The pKa of the chosen buffer should be close to the optimal pH for the biochemical reaction.
e. The chosen buffer should have a much higher concentration of its protonated species compared to the concentration of its deprotonated species at the optimal pH for the biochemical reaction.
Chemistry
1 answer:
JulsSmile [24]3 years ago
7 0

Answer:

The correct answer is: d. The pKa of the chosen buffer should be close to the optimal pH for the biochemical reaction.

Explanation:

The buffer resist or maintain the change in pH in case of Acid or basic addition to the solution. The buffer capacity should be within one or two pH units when compared to the optimal pH.

Thus it is important to select a buffer with pKa close to the optimum pH of the reaction because the ability for the buffer to maintain the pH is is great at the pH close to pKa.

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Pb3(po4)2 compound name
ch4aika [34]

Answer:

Lead phosphate

Explanation:

8 0
2 years ago
Read 2 more answers
A. Reaction with what gas in the atmosphere makes natural rain water slightly acidic? (2 points)
Vlada [557]

Answer:

See explanation

Explanation:

On the pH scale acidity ranges from pH values of 0-6.9. Natural rain water is slightly acidic due to the presence of carbon dioxide in the atmosphere which is an anhydride of carbonic acid.

CO2(g) + H2O(l) -------> H2CO3(aq)

B. The equations that turn sulfur to sulfuric acid are;

S(l) + O2(g) -----> SO2(g)

2SO2(l) + O2(g) -----> 2SO3(g)

SO3(g) + H2SO4(l) -----> H2S2O7(l)

H2S2O7(l) + H2O(l) ----> 2H2SO4(l)

-

At pH 6, H^+ concentration = 1 * 10^-6 M

At pH 2, H^+ concentration = 1 * 10^-2 M

Hence;

1 * 10^-2/1 * 10^-6

= 10^4 times more acidic

5 0
3 years ago
Which ions aren't shown in net ionic reactions, because they are present as both reactants and products of the reaction, so they
Anvisha [2.4K]

Explanation:

This is correct!

Ions that exist in both the reactant and product side of the equation are referred to as spectator ions. Overall, they do not partake in the reaction. If they are present on both sides of the equation, you can cancel them out.

An example is;

Na+(aq) + Cl​−​​(aq) + Ag​+(aq) + NO​3​−​​(aq) → Na​+​​(aq) + NO​3​−​​(aq) + AgCl(s)

The ions; Na+, NO​3​−​​(aq) would be cancelled out to give;

Cl​−​​(aq) + Ag​+(aq)  → AgCl(s)

7 0
2 years ago
Pls Help! one multiple-choice question for 13 points and brainliest!
goblinko [34]

Answer:

B

Explanation:

They use energy from sunlight to synthesise glucose from carbon dioxide and water.  

6 0
2 years ago
For molecules with more than one ionizable groups, such as glycine, which of the following statements is true? For molecules wit
tester [92]

Answer: Option (a) is the correct answer.

Explanation:

The isoelectric point (pI) is actually the pH where a particular amino acid exists as a neutral molecule. This means that if pH equals pI then there will be no electric charge on the molecule.

Therefore, the statement at pH near the pI, nearly all the molecules carry no net charge, is true.

A buffer is composed of a solution of weak acid or base and salt of weak acid/base.So, when pH equals pI then there occurs no net charge. Hence, there will be no existence of conjugate acid-base pair.

Therefore, the statement when the pH is near the pI, the solution is near its maximum buffering ability, is false.

It is known that when pH is less than pI then there will be a net positive charge on the amino acid.

Hence, the statement at a pH near the pI, nearly all the molecules carry a net positive charge, is false.

When pH is greater than pI then amino acid will have a net negative charge. Hence, the statement at a pH near the pI, nearly all the molecules carry a net negative charge, is false.

Thus, we can conclude that out of the given options the statement at pH near the pI, nearly all the molecules carry no net charge, is true.

5 0
3 years ago
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