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Answer:
There are different methods of enumeration of bacteria like viable plate count, direct microscopic method, turbidity method, etc. These methods are used to find out the number and concentration of microorganisms in any sample.
In real like the application of using enumeration methods can be seen in diary microbiology, food, microbiology, water microbiology, etc. In these sectors, the enumeration method is used to find out the quality of the food items, water.
Water quality is checked by enumerating the number of coliform bacteria by serial dilution method. In food, bacteria that cause foodborne illnesses are enumerated by using the enumeration method.
A limiting factor is anything that constrains a population's size and slows or stops it from growing. these factors are mostly biotic . For example, Food or mates. Even competing for resources. Factors for the rate of photosynthesis range. The rate of oxygen output, carbon dioxide uptake, carbohydrate (glucose/sugar) production, temperature is also a limiting factor because At low temperatures, the rate of photosynthesis is limited by the number of molecular collisions between enzymes and substrates. At high temperatures, enzymes are denatured. As well as light intensity because without the sun it’s not even possible
<span>C. Theyvare always multicellular </span>