0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
Learn more about dilution at: brainly.com/question/24881505
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Answer:
The term Big Bang defines that the Universe has expanded from the ancient hot and thick primary situation at some fixed time in the past.
Big Bang theory refers to the origin of the Universe. In the beginning, universe is very heavy and small hot ball of matter. It was a point no bigger than the head of a pin when all the things squished with each other and universe is expanded with huge explosion of energy. Due to expansion, it is cooled and results in the formation of Stars and galaxies. After sometime, atoms formed like helium and hydrogen.
Thus, statement (1), (3) and (4) is correct whereas statement (2) and (5) is incorrect. the right answer is - (It expanded slowly), (Stars and galaxies formed,) (Atoms such as hydrogen and helium formed.)
Explanation:
I would have to say cholesterol
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