Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
In cell biology, the cytoplasm is the material or protoplasm within a living cell, excluding the cell nucleus. It comprises cytosol (the gel-like substance enclosed within the cell membrane) and the organelles – the cell's internal sub-structures. All of the contents of the cells of prokaryote organisms (such as bacteria, which lack a cell nucleus) are contained within the cytoplasm. Within the cells of eukaryote organisms the contents of the cell nucleus are separated from the cytoplasm, and are then called thenucleoplasm. The cytoplasm is about 80% water and usually colorless.[1]
It is within the cytoplasm that most cellular activities occur, such as many metabolic pathways including glycolysis, and processes such as cell division. The concentrated inner area is called the endoplasm and the outer layer is called the cell cortex or theectoplasm.
Movement of calcium ions in and out of the cytoplasm is a signaling activity for metabolic processes.[2]
In plants, movement of the cytoplasm around vacuoles is known as cytoplasmic streaming.
Answer:
Molar mass
Explanation:
This is a counting unit which represents the mass in grams of a substance that make up one mole of the substance. This mass is calculated as follows:
Molar mass = Mass/ Number of moles
Units: grams/mol
Answer:
Basically an object in motion or at rest will remain that way unless an outside force acts against it.
Explanation:
Newton's first law states that every object will remain at rest or in uniform motion in a straight line unless compelled to change its state by the action of an external force. This is normally taken as the definition of inertia.
