1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
gtnhenbr [62]
3 years ago
10

Homologous recombination can be used in the lab to replace a gene of interest with a selection marker. To confirm that the selec

tion marker is integrated into the correct place in the genome, sometimes a negative selection is used in addition to the standard positive selection marker. Which of the following would act as a negative selection marker?
a. Transformation of a different gene, which does not act as a selection marker, to replace the gene of interest.â
b. A second marker in the knock out cassette, that if inserted into the genome results in cell death when plated on selective media.
c. Transforming the cells with two different knock out cassettes, each containing the same selection marker3. â
Biology
1 answer:
Luden [163]3 years ago
8 0

Answer:

b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.

Explanation:

General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.

You might be interested in
An enzyme is used as aln)
Novay_Z [31]

I'd say protein synthesizer.

5 0
3 years ago
Read 2 more answers
Studying the Mid-Atlantic Ridge showed evidence that the earths polarity reverses.
gavmur [86]

It's a the answer is true

3 0
3 years ago
Do all mutations result in negative effects? Why or why not?
Sauron [17]

Answer:

No, mutations do not all result in negative effects. Sometimes they can be beneficial, as they are completely random. It is our general tendency to think of the word "mutation" in negative terms; however, do not get confused. Mutations are the root/ultimate source of genetic variation; they are one of the main factors that allow populations to evolve.

6 0
3 years ago
Sem 1
gizmo_the_mogwai [7]

Since Science is all about experiment and reasoning, ' Which kind of milk tastes the best?' and ' Is it ethical to raise cows to produce milk?' - these two questions cannot be answered through science.

<h3>What Is Science?</h3>
  • Science is a methodical endeavor that creates and arranges knowledge in the form of universe-related explanations and predictions that can be put to the test. Some of the earliest archeological evidence for scientific reasoning is tens of thousands of years old, and science may be as old as the human species.
  • Society values science because it may be used to better living conditions and meet a wide range of fundamental human needs. Just two current examples include the search for a cancer cure and a clean source of energy.

To know more about Science please click here : brainly.com/question/17216882

#SPJ9

5 0
2 years ago
Which of the following is the best way for humans to use a seed bank to help prevent the problem
Fofino [41]
The answer is C because storing the seeds incase of famine wld prove 2 b useful.
3 0
3 years ago
Other questions:
  • 3. What type of tissue actually moves the chicken wing? <br><br> PLEASE ANSWER ASAP
    13·2 answers
  • Explain what happened genetically in the insect population over time as the insects were exposed to pyrethroids. A) The toxicity
    8·2 answers
  • How does the autonomic nervous system differ from the somatic nervous system?
    9·1 answer
  • The decimal reduction time for endospores of Bacillus cereus in milk is 36.2 minutes at 95°C. How long should it take to reduce
    9·1 answer
  • Olfactory information is sent to all of the following areas except the __________.
    14·1 answer
  • Glycolysis and the citric acid cycle comprise two different sets of oxidation reactions. thereaction sequence for glycolysis is
    6·1 answer
  • What is the ocean bottom like along the coastline?
    9·1 answer
  • Based on the information provided in the map, propose a way cholera may be transmitted.
    12·1 answer
  • Determining the order of events and the relative age of rocks by examining the position of rocks in a sequence is called____
    15·2 answers
  • Evidence for evolution includes all of the following exept
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!