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Savatey [412]
3 years ago
11

A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and tra

nsfered to a nitrocellulose membrane. The antibody reagent box in their lab contains Mouse anti-Shh MAb and Chicken anti-mouse IgG-HRP. What substrate will they need in order to perform colorimetric detection of Shh on their western blot
Biology
1 answer:
zalisa [80]3 years ago
6 0

Answer:

BCIP/NBT

Explanation:

BCIP/NBT is a colorogenic substrate used for AP. AP stands for alkaline phosphatase. When they are catalyzed with this alkaline, we would have a product that has a dark blue or probably a purple color. NBT stands for nitro blue tetrazolium. Both of these work as substrate for the reaction. BCIP oxidizes BCIP to form this color.

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Answer:

b. intercalated discs connect the muscle fibers together.

Explanation:

Intercalated discs are components of the muscles found in the heart(cardiac muscles).

Intercalated discs join the cardiomyoctes (muscle cells or fibers) together so that they can function as one and transmit signals amongst themselves during the contraction of the heart.

Intercalated discs are made of up desmosomes and gap junctions.

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Answer:

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