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Savatey [412]
2 years ago
11

A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and tra

nsfered to a nitrocellulose membrane. The antibody reagent box in their lab contains Mouse anti-Shh MAb and Chicken anti-mouse IgG-HRP. What substrate will they need in order to perform colorimetric detection of Shh on their western blot
Biology
1 answer:
zalisa [80]2 years ago
6 0

Answer:

BCIP/NBT

Explanation:

BCIP/NBT is a colorogenic substrate used for AP. AP stands for alkaline phosphatase. When they are catalyzed with this alkaline, we would have a product that has a dark blue or probably a purple color. NBT stands for nitro blue tetrazolium. Both of these work as substrate for the reaction. BCIP oxidizes BCIP to form this color.

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What did Jean-Baptiste Lamarck believe about traits such as large muscles that are acquired during one's lifetime?
Kipish [7]

<span>The answer is they can be passed on to offspring.</span>

<span>
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<span>In his theory, Lamarck proposed how species adapted to the changed environment by passing down acquired physical traits to the next generation. But, after Charles Darwin published his theory of evolution, Lamarck's theory was rejected. Now, it is known that acquired physical traits could not be inherited and epigenetics is a good proof for that.</span>

8 0
2 years ago
DRY MASS OF CORN SEEDLINGS GROWN UNDER DIFFERENT CONDITIONS
PIT_PIT [208]

Answer:

See the answer below

Explanation:

(a) One of the inorganic sources of new plant mass for the light-grown plants is the atmosphere. The <u>carbon in the form of carbon dioxide in the atmosphere is sequestrated into the body of the light-grown plants through a process known as photosynthesis</u>. During this process, the inorganic carbon dioxide becomes fixed into the body of the plant through a series of steps that include both light-dependent and light-independent. The overall equation of the process is as below:

6CO_2 + 6H_2O + Light --> C_6H_1_2O_6 + 6O_2

As more plant mass is being gained by photosynthesis, some of the gained mass is also being lost to energy generation through a process known as respiration.<u> Respiration is like the complete opposite of photosynthesis but a gain in mass is still possible because the rate of photosynthesis is more than the rate of respiration in the light-grown plants.</u>

(b) The chemical reaction that can be held responsible for the decrease in mass of the dark-grown plants is<u> respiration</u>. Some of the mass is lost to energy generation and this mass cannot be recouped by adequate photosynthesis due to light limitation. The energy is needed to metabolic processes that would keep the plants alive. The equation of respiration is as below:

C_6H_1_2O_6 + 6O_2 --> 6CO_2 + 6H_2O + Energy

6 0
2 years ago
4. Two patchwork fish are crossed. What is the probability that they will have patchwork fish?
schepotkina [342]

50% it said i need more words hope this helps have a nice day.

4 0
2 years ago
Does the death of an organism result in increased or decreased entropy
Anastasy [175]

Answer;

Increased

Death of an organism results to an increased entropy because as soon as an organism dies, another one is formed.

Explanation;

Entropy  is the measure of the unusable energy that escapes when energy is being converted.

The 'heat-death' of the universe is when the universe has reached a state of maximum entropy. This happens when all available energy (such as from a hot source) has moved to places of less energy (such as a colder source). Once this has happened, no more work can be extracted from the universe. Since heat ceases to flow, no more work can be acquired from heat transfer.

6 0
3 years ago
What happens to reactant molecules when forming product molecules?
katen-ka-za [31]
<span>The reactants are any substance, elements or compounds in chemical reactions.
So now, the question is what will happen to the reactants molecules when combined to the product molecules? The reactant molecules will be divided or split and will be merge with other molecule to form a new substance called a product molecule.
For example: The combination of gas substances like Methane and Oxygen (reactants) to carbon dioxide and water (products). Together they will form a new product molecule.</span>



6 0
3 years ago
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