A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and tra

Question

3 years ago

11

A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and tra

nsfered to a nitrocellulose membrane. The antibody reagent box in their lab contains Mouse anti-Shh MAb and Chicken anti-mouse IgG-HRP. What substrate will they need in order to perform colorimetric detection of Shh on their western blot

Biology

1 answer:

zalisa [80] · Answer 1 · 2022-07-15T09:19:24+03:00

Answer:

BCIP/NBT

Explanation:

BCIP/NBT is a colorogenic substrate used for AP. AP stands for alkaline phosphatase. When they are catalyzed with this alkaline, we would have a product that has a dark blue or probably a purple color. NBT stands for nitro blue tetrazolium. Both of these work as substrate for the reaction. BCIP oxidizes BCIP to form this color.