Answer:
High concentration of glutathione should be included in the elution buffer for the given experiment to remove protein from the column.
Explanation:
Affinity chromatography is one of important biochemical technique of chromatography which depends on the affinity of ligand for the receptor.Here the ligand is a protein mixture which act as mobile phase and the receptor is present in the wall of chromatography column act as stationary phase.When the protein mixture is applied on the top of the column the substances present within the protein that have high affinity for the receptor present in the walls of chromatography column binds to the later but rest of the protein pass away through the column.High concentration of ligand is used within an buffer solution to remove the desired protein from the column.
From this point of view it can be stated that in the given question high concentration of glutathione should be used to remove the desired protein from the column.
In the so called rain shadow effect we have interaction between all of the four major Earth spheres. When we have a coastal region where there's a high mountain range, the part of the mountain that is facing the sea will differ a lot from the part of the mountain that is on the other side. The water from the sea evaporates. The water vapor makes the air wet. The warm and wet air masses from the sea will come to the coastline, once they reach the mountain they will start to accumulate as they can not pass through it. As they accumulate rainfall appears. The rainfall contributes to a lush vegetation on this side of the mountain (windward side). The rain shadow effect appears on the leeward side of the mountain, and it mostly gets dry, strong, downward winds. These conditions result in drier climate, much less vegetation, and much increased erosion. Thus we can easily see that we have in this case interaction between the hydrosphere (the sea and the rainfall), the geosphere (the ground, soil, rocks), biosphere (the vegetation), and atmosphere (the winds, the clouds).
