Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
“The nth term of the following types of sequences can be found out using the following Formulas:
1.Arithmetic Progression(AP) : here, the consecutive terms differ by a common difference (d).
tn= a+(n-1)d
Where,
a= is the first term of the sequence,
n= nth term
d= common difference
2. Geometric Progression(GP):
Here, the consequtive terms differ by a common ratio (r).
Tn= ar^(n-1)
where,
a = first term
r = common ratio
tn= nth term
3.Harmonic Progression (H.P):
It is the reciprocal of AP.
To find nth term of a HP, first convert it into AP and then apply the tn formula for the sequence and take the reciprocal of the obtained tn again.
Hope the answer helps you!!”
Human exploitation of fragile ecosystems can lead to the droughts and arid conditions characteristic of desertification. Effects include land degradation, soil erosion and sterility, and a loss of biodiversity, with huge economic costs for nations where deserts are growing.
The Aromatic hydrocarbon is the most common in nature.
One reasonable plan would be to wear a face mask or surgical
mask. Take note that your surgical mask contains two sides – the white side and the
blue side. Since the main purpose of your mask usage is to prevent yourself
from inhaling too much bad air, the blue side of the mask should be the one
touching your skin. This is because the white side of your mask helps you
prevent from coming in contact with toxins that may come from the smog.
