To solve this problem,
we can use the Henderson-Hasselbalch Equation which relates the pH to the measure
of acidity pKa. The equation is given as:<span>
<span>pH = pKa + log ([base]/[acid]) ---> 1</span></span>
Where,
[base] = concentration
of C2H3O2
in molarity or moles
<span>[acid] = concentration of HC2H3O2 in molarity or moles</span>
For the sake of easy calculation, let us assume that:
[base] = 1
[acid] = x
<span>
Therefore using equation 1,
4.24 = 4.74 + log (1 / x)
<span>log (1 / x) = - 0.5
1 / x = 0.6065 </span></span>
x =
1.65<span>
The required ratio of C2H3O2 /HC2H3O2 <span>
is 1:1.65 or 3:5. </span></span>
<span>The following is the role of NaCl and EDTA in DNA isolation, hope it helps:
NaCl provides Na+ ions that will block negative charge from phosphates on DNA.
Negatively charged phosphates on DNA cause molecules to repel each other. The Na+ ions will form an ionic bond with the negatively charged phosphates on the DNA, neutralizing the negative charges and allowing the DNA molecules to come together.</span>
What is it you need help on? there is nothing here????
ANSWER:
The molecular formula :
C₆H₁₄O₃PF
<h3>Further explanation</h3>
Given
39.10% carbon, 7.67% hydrogen, 26.11% oxygen, 16.82% phosphorus, and 10.30% fluorine.
Required
The molecular formula
Solution
mol ratio :
C = 39.1 : 12 = 3.258
H = 7.67 : 1 = 7.67
O = 26.11 : 16 = 1.632
P = 16.82 : 31 = 0.543
F = 10.3 : 19 = 0.542
Divide by 0.542
C = 6
H : 14
O = 3
P = 1
F = 1
The empirical formula :
C₆H₁₄O₃PF
(The empirical formula)n = the molecular formula
(C₆H₁₄O₃PF)=184.1
(6.12+14.1+3.16+31+19)n=184.1
(184)n=184.1
n = 1
