1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
zlopas [31]
3 years ago
10

You have selected your two primers and added all of the PCR components to a test tube. Use the answer choices [A-C] to explain w

hat will happen as the polymerase chain reaction proceeds. Answers may be used more than once or not at all.
Chemistry
1 answer:
tankabanditka [31]3 years ago
7 0

Hi. You did not provide any response options. However, a PCR reaction proceeds as follows.

After the primers are added to the test tube containing the PCR components. This tube is placed in a device called a thermocycler. At that moment, the stage called denaturation will begin, where the thermocycler increases the temperature to the point of breaking the hydrogen bonds that hold the two strands of DNA together. The thermal cycler increases the temperature up to 96°C.

After that, the second step of the reaction begins. At that moment, the thermal cycler lowers the temperature to 55º - 65ºC, which is the ideal temperature for the primers to be able to attach themselves to the DNA strands, preparing them for the presence of the polymerase.

After that, the thermocycler raises the temperature to 72ºC, which is the ideal temperature for the DNA polymerase to work. At this stage, the DNA polymerase will use the DNA strand and the primer to build a new DNA strand, which will be annealed to the DNA strand used as a template.

These three steps will be repeated about 35 times, generating many copies of DNA.

You might be interested in
Smallest to largest: biome,population,organism,ecosystem,community
Ivahew [28]
Organism, population, community, ecosystem, and biome
7 0
3 years ago
What is the percentage by mass of sulphur in Al2(SO4)3[A12SO4 =<br>342g/mol, S = 32]​
valentinak56 [21]

Answer: The percentage by mass of sulphur in Al_2(SO_4)_3 is 9.36%

Explanation:

Mass percent of an element is the ratio of mass of that element by the total mass expressed in terms of percentage.

{\text {Mass percentage}}=\frac{\text {mass of sulphur}}{\text {Total mass}}\times 100\%

Given: mass of sulphur = 32 g/mol

mass of Al_2(SO_4)_3 = 342 g/mol

Putting in the values we get:

{\text {Mass percentage}}=\frac{32g/mol}{342g/mol}\times 100\%=9.36\%

The percentage by mass of sulphur in Al_2(SO_4)_3 is 9.36%

4 0
3 years ago
The Olympian swam 200 meters in 20 seconds. <br> 1) velocity<br> 2) acceleration<br> 3) speed
irakobra [83]

Answer: velocity

Explanation:

3 0
2 years ago
Read 2 more answers
PLZ HELP!<br><br><br>How does changing the angle of a ramp affect the force required?
galina1969 [7]

Answer:

depending on how you change the angle on the ramp the kanetic energy can go up or down depending on how u angle the ramp

Explanation:

7 0
2 years ago
Make a checklist of eight good characteristics of a experiment.
natka813 [3]
I could only find 7!

- independent variable
- dependent variable
- control group
- experimental group
- constant
- observation
- inference
6 0
3 years ago
Other questions:
  • How does the periodic table indicate the number of valence electrons element has?
    14·1 answer
  • What are is the pH range of phenolphthalein and methyl orange​
    8·1 answer
  • _Cuo +H, → _Cu + _H,0
    15·1 answer
  • The air pressure in a tire is 2.38 atm. What is the pressure in kilopascals.
    7·1 answer
  • How many molecules are in 4.545 moles of carbon dioxide (CO2)
    13·1 answer
  • Consider 3.01*10^24 molecules of oxygen (atomic mass is 16.0 g/mole) in a container at a temperature of 22.0 degrees celsius. If
    11·1 answer
  • 2 A + 4 B → 2 C How many mol of B must react with excess A to produce 2 mol of C?
    15·1 answer
  • An atom of carbon contains 6 total electrons. How many of these electrons are in s orbitals?
    14·1 answer
  • Draw the structures of the aldol addition and condensation products of cyclopentanone
    13·1 answer
  • How many neutrones does the isotope n-14 have
    10·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!