IV only
Plate IV will only have the trangenic E Coli hence will have the highest percentage of bacteria that are expected to produce insulin.
Explanation:
The transgenic <em>E. coli </em>will have taken up the plasmid with the insulin and <em>amp</em> genes hence they will be able to survive and grow in amplicilin antibiotic.
In plate IV, the wild type <em>E. coli</em> will be eliminated by the antibiotic (because they do not have the gene for resistance) hence providing no competition for resources, in the agar, with the transgenic <em>E coli</em>. This will ensure optimal growth for the transgenic <em>E coli</em> even, than in plate III, producing highest percentage of insulin.
Learn More:
For more on gene cloning check out;
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.c.No. The mutation occurred in skin cells and not the ovaries, where egg cells are generated
For the answer to the question above, one of the side effects of carbon in the environment is the global warming or Climate change. The Carbon dioxide traps the heat of the sun from escaping the atmosphere. This results to increase in global temperature which causes the ocean water to evaporate than usual and creates super typhoons.