Back titration experiment
1 answer:
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Answer:
The barrier has to be 34.23 kJ/mol lower when the sucrose is in the active site of the enzyme
Explanation:
From the given information:
The activation barrier for the hydrolysis of sucrose into glucose and fructose is 108 kJ/mol.
In this same concentration for the glucose and fructose; the reaction rate can be calculated by the rate factor which can be illustrated from the Arrhenius equation;
Rate factor in the absence of catalyst:
Rate factor in the presence of catalyst:
Assuming the catalyzed reaction and the uncatalyzed reaction are taking place at the same temperature :
Then;
the ratio of the rate factors can be expressed as:
Thus;
Let say the assumed temperature = 25° C
= (25+ 273)K
= 298 K
Then ;
The barrier has to be 34.23 kJ/mol lower when the sucrose is in the active site of the enzyme