Before the development of electrophoresis to separate macromolecules, high-speed centrifugation was used to isolate DNA.
A laboratory procedure called electrophoresis is used to divide DNA, RNA, or protein molecules according to their size and electrical charge. The molecules are moved by an electric current through a gel or other matrix. The technology of electrophoresis is crucial for the separation and examination of nucleic acids. At the lab bench, cloned DNA fragments are frequently isolated and worked with using nucleic acid electrophoresis.
High-speed centrifugation employs centrifugal force to separate particles with various densities or masses suspended in a liquid. High-speed rotation of the solution inside the tube causes each particle's angular momentum to experience centrifugal forces inversely proportionate to its mass.
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1, 6, 2, 6
In the order you wrote them in
Molarity is defined as the number of moles of solute in 1 L of solution
molar mass of Ni(NO₃)₂ - 182.7 g/mol
number of moles of Ni(NO₃)₂ - 5.80 g/ 182.7 g/mol = 0.0317 mol
number of moles in 500 mL - 0.0317 mol
therefore number of moles in 1000 mL - 0.0317 mol / 500 mL x 1000 mL/L
molarity of the solution is - 0.0634 M
The reaction is extremely exothermic, producing a bright yellow light and a great deal of heat energy.
