Answer:
I think its 2 mass correct me if im wrong but I'm very sure
Answer:
DNA to messenger RNA (mRNA) to protein.
Explanation:
Central Dogma is a term that refers to the two step process through which information flows from the sequence of DNA into a sequence of RNA (the process of transcription) and finally takes the form of proteins (the process of translation)
Francis Crick was the first scientist who proposed the term of "Central Dogma".
Significance of Central Dogma:
- This is the process that ensures that genetic information of all living organisms is preserved and transmitted without any alteration or changes into their offspring.
- It is a process that intricately explains the logical mechanism through which DNA stored in our chromosomes express themselves into functional forms called proteins.
- This was the process which let us know the important roles of molecules like ribosomal RNA, transfer RNA and messenger RNA in the process of protein formation.
- The process has immense importance in understanding the latest advances in RNA technology that can further help in investigation and diagnosis as well as treatment of many critical diseases.
Hope it helps!
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
The human respiratory system contains the organs that allow us to get the oxygen we need and to remove the waste carbon dioxide we do not need. It contains these parts: two lungs. ... various structures in the chest that allow air to move in and out of the lungs.
