It should be puberty because once a female starts her menstrual cycle she is able to become pregnant. The same should go for guys when it comes to their sperm becoming "active" in other words
By making them exercise more often
Kirby Bauer disk technique is a method to determine sensitivity of microorganisms yo antimicrobial drugs. The greater the efficacy of the chemical the larger zone of inhibition on around the drug containing disks at lowest concentration possible or the minimum inhibitory concentration. If the chemical found on the skin of frogs produce a large zone of inhibition at a very low concentration of the drug this can be a good antibiotic.
