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Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
The correct option is this: ANCESTRAL STATE RECONSTRUCTION SUGGESTING THAT THESE ANIMALS HAD OPSINS THAT WERE SHIFTED TOWARD THE RED.
Ancestral reconstruction is the extrapolation back in time from measured characteristics of individuals to their common ancestors. Opsins are a group of light sensitive proteins that are located in the photoreceptor cells of the retina of the eyes. Having opsin that were shifted toward the red colour will enable the animal to see very well in the night time, thus, the animal will be active during that period.
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Answer:
What happens during DNA replication?
How is DNA replicated? Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment. During separation, the two strands of the DNA double helix uncoil at a specific location called the origin.
Explanation:
